The impact of antidepressant on gene expression in Danio rerio embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43876969" target="_blank" >RIV/62157124:16270/18:43876969 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The impact of antidepressant on gene expression in Danio rerio embryos

Popis výsledku v původním jazyce

Zebrafish (Danio rerio) embryos in different stages of development were used for assessment of venlafaxine antidepressant impact on non-target organisms. Two different concentrations of venlafaxine were chosen, the 300 ng/l representing the environmental concentrations (low) and the 30 ?g/l as 100x higher (high) concentration for evaluation of dose depending effect. For the gene expression, CYP1A and GST gene and two multidrug resistance proteins coded by genes ABCC1 and ABCB4 gene were selected, each of them representing one of the phase zero to III of biotransformation. The results of assay showed that the impact of venlafaxine on the zebrafish embryos is the most evident in the time of hatching (96 hours post fertilization). In this time, the results of gene expression showed increase in mRNA amount of ABCB4 and GST in both concentrations of venlafaxine. The CYP1A and ABCC1 gene revealed increase in high concentration of venlafaxine; in contrast, the low concentration for these genes revealed the decrease in mRNA amount. The second increase in gene expression was observed in 144 hours post fertilization for both concentrations of venlafaxine and all genes. The study showed that venlafaxine can affect the gene expression of biotransformation enzymes of Danio rerio embryos already in environmental concentration.

Název v anglickém jazyce

The impact of antidepressant on gene expression in Danio rerio embryos

Popis výsledku anglicky

Zebrafish (Danio rerio) embryos in different stages of development were used for assessment of venlafaxine antidepressant impact on non-target organisms. Two different concentrations of venlafaxine were chosen, the 300 ng/l representing the environmental concentrations (low) and the 30 ?g/l as 100x higher (high) concentration for evaluation of dose depending effect. For the gene expression, CYP1A and GST gene and two multidrug resistance proteins coded by genes ABCC1 and ABCB4 gene were selected, each of them representing one of the phase zero to III of biotransformation. The results of assay showed that the impact of venlafaxine on the zebrafish embryos is the most evident in the time of hatching (96 hours post fertilization). In this time, the results of gene expression showed increase in mRNA amount of ABCB4 and GST in both concentrations of venlafaxine. The CYP1A and ABCC1 gene revealed increase in high concentration of venlafaxine; in contrast, the low concentration for these genes revealed the decrease in mRNA amount. The second increase in gene expression was observed in 144 hours post fertilization for both concentrations of venlafaxine and all genes. The study showed that venlafaxine can affect the gene expression of biotransformation enzymes of Danio rerio embryos already in environmental concentration.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Water &amp; Fish 8th International Conference

ISBN

978-86-7834-308-7

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

181-185

Název nakladatele

Faculty of Agriculture, University of Belgrade, Serbia

Místo vydání

Belgrade

Místo konání akce

Bělehrad

Datum konání akce

13. 6. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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