Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877923" target="_blank" >RIV/62157124:16270/19:43877923 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/19:73599762 RIV/62157124:16810/19:43877923

Výsledek na webu

<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/lam.13121" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/lam.13121</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/lam.13121" target="_blank" >10.1111/lam.13121</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic

Popis výsledku v původním jazyce

Vancomycin-resistant enterococci (VRE) are nosocomial pathogens of increasing medical importance. This study involved 121 VRE selectively obtained from a representative set of 1464 samples collected from various sources in the north-eastern part of the Czech Republic. In total, 119 VRE belonged to Enterococcus faecium and two to Enterococcus faecalis. All isolates of E. faecium were resistant to at least three antibiotic classes. The resistance genes vanA, erm(B), tet(M), tet(L), aac(3)-IIIa and aac(6 &apos;)-aph(2 &apos;&apos;) were detected. We assigned the E. faecium to sequence types ST5, ST18, ST38, ST64, ST92, ST273, ST549 and ST640. In E. faecium isolates, we identified the presence of replicases rep20(pLG1), rep2(pRE25), rep17(pRUM), rep21(pVEF1/2) and rep14(pRI1), as well as relaxases rel(pEF1), rel(pLG1), rel(pCIZ2), rel(pRE25) and rel(pRUM). The presence of the toxin-antitoxin system axe-txe was detected mainly among isolates of hospital origin. The A and D types of transposon Tn1546 were those occurring most frequently. Significance and Impact of the Study This is the first extensive study of vancomycin-resistant enterococci of diverse origin in a single well-defined area of the Czech Republic. The isolates were investigated for their antibiotic resistance, epidemiological characteristics and plasmid characteristics. Based on the results obtained, we can make assumptions as to the ways that vancomycin resistance is disseminated throughout the environment including humans and animals.

Název v anglickém jazyce

Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic

Popis výsledku anglicky

Vancomycin-resistant enterococci (VRE) are nosocomial pathogens of increasing medical importance. This study involved 121 VRE selectively obtained from a representative set of 1464 samples collected from various sources in the north-eastern part of the Czech Republic. In total, 119 VRE belonged to Enterococcus faecium and two to Enterococcus faecalis. All isolates of E. faecium were resistant to at least three antibiotic classes. The resistance genes vanA, erm(B), tet(M), tet(L), aac(3)-IIIa and aac(6 &apos;)-aph(2 &apos;&apos;) were detected. We assigned the E. faecium to sequence types ST5, ST18, ST38, ST64, ST92, ST273, ST549 and ST640. In E. faecium isolates, we identified the presence of replicases rep20(pLG1), rep2(pRE25), rep17(pRUM), rep21(pVEF1/2) and rep14(pRI1), as well as relaxases rel(pEF1), rel(pLG1), rel(pCIZ2), rel(pRE25) and rel(pRUM). The presence of the toxin-antitoxin system axe-txe was detected mainly among isolates of hospital origin. The A and D types of transposon Tn1546 were those occurring most frequently. Significance and Impact of the Study This is the first extensive study of vancomycin-resistant enterococci of diverse origin in a single well-defined area of the Czech Republic. The isolates were investigated for their antibiotic resistance, epidemiological characteristics and plasmid characteristics. Based on the results obtained, we can make assumptions as to the ways that vancomycin resistance is disseminated throughout the environment including humans and animals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Letters in Applied Microbiology

ISSN

0266-8254

e-ISSN

—

Svazek periodika

69

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

16-22

Kód UT WoS článku

000471301500003

EID výsledku v databázi Scopus

2-s2.0-85067239663