Molecular characterization of Aeromonas spp. isolates carrying plasmid-mediated quinolone resistance genes from carp and rainbow trout fish in Czech Republic

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of Aeromonas spp. isolates carrying plasmid-mediated quinolone resistance genes from carp and rainbow trout fish in Czech Republic

Popis výsledku v původním jazyce

Quinolone resistance in bacteria have been reported on a global scale and it is also associated with aquatic environments, prominently found in aeromonads. The aim of this study was to isolate and characterize Aeromonas spp. isolates with reduced susceptibility to ciprofloxacin in food fish (carp and rainbow trout fish) bred in the Czech Republic, and to assess their potential risks as sources of plasmid-mediated resistance to fluoroquinolones. Thirty six qnrS2-positive Aeromonas spp. isolates were speciated by sequencing of rpoB gene. Isolates originating from carps belonged to Aeromonas veronii while isolates from rainbow trout were identified as Aeromonas salmonicida. Susceptibility testing revealed that all selected isolates were resistant to oxolinic acid with a majority of isolates resistant to fluemequine and oxytetracycline. PCR-based detection showed that at least 2 virulence genes were associated with these isolates. Whole genome sequencing revealed that qnrS2 gene was located within a mobile insertion cassette and carried by a small mobilizable plasmid or larger IncU plasmids. A. veronii had low virulence with high diversity of genetic profile while A. salmonicida had at least 5 virulence genes and belonged to sequence type (ST)2. This study revealed the dissemination of transferrable quinolone resistance in fish pathogens in Czech fisheries.

Název v anglickém jazyce

Molecular characterization of Aeromonas spp. isolates carrying plasmid-mediated quinolone resistance genes from carp and rainbow trout fish in Czech Republic

Popis výsledku anglicky

Quinolone resistance in bacteria have been reported on a global scale and it is also associated with aquatic environments, prominently found in aeromonads. The aim of this study was to isolate and characterize Aeromonas spp. isolates with reduced susceptibility to ciprofloxacin in food fish (carp and rainbow trout fish) bred in the Czech Republic, and to assess their potential risks as sources of plasmid-mediated resistance to fluoroquinolones. Thirty six qnrS2-positive Aeromonas spp. isolates were speciated by sequencing of rpoB gene. Isolates originating from carps belonged to Aeromonas veronii while isolates from rainbow trout were identified as Aeromonas salmonicida. Susceptibility testing revealed that all selected isolates were resistant to oxolinic acid with a majority of isolates resistant to fluemequine and oxytetracycline. PCR-based detection showed that at least 2 virulence genes were associated with these isolates. Whole genome sequencing revealed that qnrS2 gene was located within a mobile insertion cassette and carried by a small mobilizable plasmid or larger IncU plasmids. A. veronii had low virulence with high diversity of genetic profile while A. salmonicida had at least 5 virulence genes and belonged to sequence type (ST)2. This study revealed the dissemination of transferrable quinolone resistance in fish pathogens in Czech fisheries.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ, Sborník příspěvků

ISBN

978-80-7305-820-3

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

71-73

Název nakladatele

Veterinární a farmaceutická univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

22. 5. 2019

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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