Plasmids in pathogenic multiresistant Escherichia coli

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878236" target="_blank" >RIV/62157124:16270/19:43878236 - isvavai.cz</a>

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RIV/62157124:16810/19:43878236

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasmids in pathogenic multiresistant Escherichia coli

Popis výsledku v původním jazyce

Even though Escherichia coli is a gastrointestinal tract commensal, ExPEC strains (extraintestinal pathogenic E. coli) represent a serious issue to public health. Plasmids contribute to the successful dissemination of the ExPEC strains thanks to many virulence and antibiotic resistance genes they carry. In our recent study we showed that some sublineages of E. coli ST131 obtained from diverse sources including humans, animals and waste water contained specific plasmids from incompatibility group F (IncF). These plasmids have mosaic structure, they contain often more than one IncF plasmid replicon, and encode blaCTX-M genes that allows extended-spectrum beta-lactamases production. The focus of this study is to better understand the impact of plasmids on the host fitness and on a worldwide spread of these clinically important strains. To study the bacterial fitness in the presence and absence of plasmid we need to eliminate IncF plasmids from wild-type isolates. Plasmid-free cells will be obtained using plasmid constructs. These constructed plasmids carry IncF plasmid replicons which interfere with the replicons of wild-type IncF plasmids. We will use commercial plasmid pCURE2 and a new plasmid construct pCURE-F we designed to specifically target IncF replicons which are carried by the strains from our collection. The pCURE plasmids are then eliminated thanks to their instability in a non-selective medium. After we successfully eliminate the plasmids, further analyses, such as whole genome sequencing, growth curves and competition assays, will be done aiming to understand the impact of IncF plasmids on the host cell

Název v anglickém jazyce

Plasmids in pathogenic multiresistant Escherichia coli

Popis výsledku anglicky

Even though Escherichia coli is a gastrointestinal tract commensal, ExPEC strains (extraintestinal pathogenic E. coli) represent a serious issue to public health. Plasmids contribute to the successful dissemination of the ExPEC strains thanks to many virulence and antibiotic resistance genes they carry. In our recent study we showed that some sublineages of E. coli ST131 obtained from diverse sources including humans, animals and waste water contained specific plasmids from incompatibility group F (IncF). These plasmids have mosaic structure, they contain often more than one IncF plasmid replicon, and encode blaCTX-M genes that allows extended-spectrum beta-lactamases production. The focus of this study is to better understand the impact of plasmids on the host fitness and on a worldwide spread of these clinically important strains. To study the bacterial fitness in the presence and absence of plasmid we need to eliminate IncF plasmids from wild-type isolates. Plasmid-free cells will be obtained using plasmid constructs. These constructed plasmids carry IncF plasmid replicons which interfere with the replicons of wild-type IncF plasmids. We will use commercial plasmid pCURE2 and a new plasmid construct pCURE-F we designed to specifically target IncF replicons which are carried by the strains from our collection. The pCURE plasmids are then eliminated thanks to their instability in a non-selective medium. After we successfully eliminate the plasmids, further analyses, such as whole genome sequencing, growth curves and competition assays, will be done aiming to understand the impact of IncF plasmids on the host cell

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-23532S" target="_blank" >GA18-23532S: Multirezistentní vysoce rizikové linie Escherichia coli: příběh úspěšné souhry mezi chromozomem a plazmidem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů