IncI1 ESBL-producing plasmids from Escherichia coli obtained from patients are closely related to plasmids from E. coli of animal origin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878238" target="_blank" >RIV/62157124:16270/19:43878238 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/19:43878238

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

IncI1 ESBL-producing plasmids from Escherichia coli obtained from patients are closely related to plasmids from E. coli of animal origin

Popis výsledku v původním jazyce

Twelve E. coli isolates sharing identical ESBL genes and pMLST were sequenced on Illumina and MinION platforms. The IncI1/ST3 plasmids were obtained from ten E. coli isolates (two from patients with bloodstream infections, six from food and two from animals). The IncI1/ST7 plasmids originated from E. coli isolates from a patient with bloodstream infection and from a pig. Sequences of IncI1/ST3 and IncI1/ST7 plasmids harbouring blaCTX-M-1 retrieved from GenBank were used for comparison within the respective group. The ten IncI1/ST3 and two IncI1/ST7 plasmids carrying blaCTX-M-1 were highly similar in structure and organization with only minor rearrangements and differences in the variable region. The high level of similarity was also observed in plasmids from E. coli of animal origin from Australia, Switzerland, the Netherlands and France. This study revealed high level of similarity in organization, structure and accessory regions of IncI1/ST3 and IncI1/ST7 plasmids, respectively. These findings suggest broad dissemination of a very successful blaCTX-M-1- carrying plasmid lineages among E. coli of both human and animal origin.

Název v anglickém jazyce

IncI1 ESBL-producing plasmids from Escherichia coli obtained from patients are closely related to plasmids from E. coli of animal origin

Popis výsledku anglicky

Twelve E. coli isolates sharing identical ESBL genes and pMLST were sequenced on Illumina and MinION platforms. The IncI1/ST3 plasmids were obtained from ten E. coli isolates (two from patients with bloodstream infections, six from food and two from animals). The IncI1/ST7 plasmids originated from E. coli isolates from a patient with bloodstream infection and from a pig. Sequences of IncI1/ST3 and IncI1/ST7 plasmids harbouring blaCTX-M-1 retrieved from GenBank were used for comparison within the respective group. The ten IncI1/ST3 and two IncI1/ST7 plasmids carrying blaCTX-M-1 were highly similar in structure and organization with only minor rearrangements and differences in the variable region. The high level of similarity was also observed in plasmids from E. coli of animal origin from Australia, Switzerland, the Netherlands and France. This study revealed high level of similarity in organization, structure and accessory regions of IncI1/ST3 and IncI1/ST7 plasmids, respectively. These findings suggest broad dissemination of a very successful blaCTX-M-1- carrying plasmid lineages among E. coli of both human and animal origin.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV18-09-00605" target="_blank" >NV18-09-00605: Enterobacteriaceae rezistentní ke karbapenemům a kolistinu: koncept jednoho zdraví v hodnocení humánních a jiných zdrojů rizikových mechanizmů antimikrobiální rezistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů