Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878809" target="_blank" >RIV/62157124:16270/20:43878809 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/89/2/0151/" target="_blank" >https://actavet.vfu.cz/89/2/0151/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089020151" target="_blank" >10.2754/avb202089020151</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model

Popis výsledku v původním jazyce

Polychlorinated biphenyls (PCBs) are carcinogens causing endocrine disruption. While production of PCBs is now banned, wildlife exposure still occurs due to environmental contamination. We investigated thyroid toxicity in wild ungulates using three-dimensional primary thyrocyte cultures exposed to PCB 138 for 24, 48, and 72 h at concentrations ranging within 0-3000 ng/ml. Thyrocyte viability ranged within 78.71-118.34%, 98.14-104.45%, and 84.16-106.70% in fallow deer-, mouflon-, and roe deer-derived cells, respectively. Viability decreased significantly in fallow deer (P = 0.012) and roe deer (P = 0.002) thyrocytes exposed for 48 h at 30 ng/ml. While cytotoxicity ranged within 2.36-16.37%, 3.19-9.85%, and 2.76-11.21% in fallow deer, mouflon, and roe deer, respectively, only roe deer displayed significantly higher cytotoxicity at a 3 ng/ml exposure (P &lt; 0.05) and lower cytotoxicity at 30 ng/ml (P &lt; 0.01). Exposure to 30 ng/ml for 24 and 48 h induced reactive oxygen species in fallow deer. Iodide uptake at 30 ng/ml exposure increased after 24 h in fallow and roe deer, but showed a significant drop after 48 and 72 h in fallow deer, mouflon, and roe deer. Thyroxine T-4 release at 30 ng/ml exposure decreased significantly after 48 and 72 h; 24, 48 and 72 h; and 48 h in fallow deer, mouflon, and roe deer, respectively. Our findings indicate time- and species-dependent effects of PCB on performance and thyrocyte function. Use of cell culture models reduces the number of experimental specimens, increases test species welfare and replaces whole organisms with specific target cells.

Název v anglickém jazyce

Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model

Popis výsledku anglicky

Polychlorinated biphenyls (PCBs) are carcinogens causing endocrine disruption. While production of PCBs is now banned, wildlife exposure still occurs due to environmental contamination. We investigated thyroid toxicity in wild ungulates using three-dimensional primary thyrocyte cultures exposed to PCB 138 for 24, 48, and 72 h at concentrations ranging within 0-3000 ng/ml. Thyrocyte viability ranged within 78.71-118.34%, 98.14-104.45%, and 84.16-106.70% in fallow deer-, mouflon-, and roe deer-derived cells, respectively. Viability decreased significantly in fallow deer (P = 0.012) and roe deer (P = 0.002) thyrocytes exposed for 48 h at 30 ng/ml. While cytotoxicity ranged within 2.36-16.37%, 3.19-9.85%, and 2.76-11.21% in fallow deer, mouflon, and roe deer, respectively, only roe deer displayed significantly higher cytotoxicity at a 3 ng/ml exposure (P &lt; 0.05) and lower cytotoxicity at 30 ng/ml (P &lt; 0.01). Exposure to 30 ng/ml for 24 and 48 h induced reactive oxygen species in fallow deer. Iodide uptake at 30 ng/ml exposure increased after 24 h in fallow and roe deer, but showed a significant drop after 48 and 72 h in fallow deer, mouflon, and roe deer. Thyroxine T-4 release at 30 ng/ml exposure decreased significantly after 48 and 72 h; 24, 48 and 72 h; and 48 h in fallow deer, mouflon, and roe deer, respectively. Our findings indicate time- and species-dependent effects of PCB on performance and thyrocyte function. Use of cell culture models reduces the number of experimental specimens, increases test species welfare and replaces whole organisms with specific target cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

151-162

Kód UT WoS článku

000548359900006

EID výsledku v databázi Scopus

—