Measurement of phagocyte activity in heterotherms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878811" target="_blank" >RIV/62157124:16270/20:43878811 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/20:00523876 RIV/00216224:14310/20:00115848

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2020089010079.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2020089010079.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089010079" target="_blank" >10.2754/avb202089010079</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Measurement of phagocyte activity in heterotherms

Popis výsledku v původním jazyce

The heterotherm immune system undergoes significant variation in response to life cycle periodicity and torpor. As heterothermic bats are important reservoirs of zoonotic agents and modulation of immune activity can affect host-pathogen interactions, this work aimed at developing a suitable method for assessing heterotherm phagocyte activity. Chemiluminescence measurements were evaluated by mathematical and mechanistic approaches, both of which yielded comparable results in time-related parameters of phagocyte activity. Using a mathematical method, however, we developed a model that can be applied to particular specimens. The proposed equation offers a simple and reliable tool for comparing phagocyte activity, the values of which can be used for further analysis. While time-related parameters of bat phagocyte activity varied with measurement temperature, with the onset of respiratory burst at 38 degrees C being quicker than at 25 degrees C, quantitative values of phagocyte activity were not influenced by measurement temperature. Further, homeotherm phagocyte activity parameters were more variable at 25 degrees C. Considering there was no influence of measurement temperature on the total volume of heterotherm phagocyte activity, we suggest that parameters measured at 25 degrees C are more representative of the immune status adapted to physiological extremes at low body temperatures.

Název v anglickém jazyce

Measurement of phagocyte activity in heterotherms

Popis výsledku anglicky

The heterotherm immune system undergoes significant variation in response to life cycle periodicity and torpor. As heterothermic bats are important reservoirs of zoonotic agents and modulation of immune activity can affect host-pathogen interactions, this work aimed at developing a suitable method for assessing heterotherm phagocyte activity. Chemiluminescence measurements were evaluated by mathematical and mechanistic approaches, both of which yielded comparable results in time-related parameters of phagocyte activity. Using a mathematical method, however, we developed a model that can be applied to particular specimens. The proposed equation offers a simple and reliable tool for comparing phagocyte activity, the values of which can be used for further analysis. While time-related parameters of bat phagocyte activity varied with measurement temperature, with the onset of respiratory burst at 38 degrees C being quicker than at 25 degrees C, quantitative values of phagocyte activity were not influenced by measurement temperature. Further, homeotherm phagocyte activity parameters were more variable at 25 degrees C. Considering there was no influence of measurement temperature on the total volume of heterotherm phagocyte activity, we suggest that parameters measured at 25 degrees C are more representative of the immune status adapted to physiological extremes at low body temperatures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

79-87

Kód UT WoS článku

000529064300010

EID výsledku v databázi Scopus

—