IncX plasmids and their transferability under induced stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879712" target="_blank" >RIV/62157124:16270/21:43879712 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/21:43879712

Výsledek na webu

<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

IncX plasmids and their transferability under induced stress

Popis výsledku v původním jazyce

Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.

Název v anglickém jazyce

IncX plasmids and their transferability under induced stress

Popis výsledku anglicky

Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-23532S" target="_blank" >GA18-23532S: Multirezistentní vysoce rizikové linie Escherichia coli: příběh úspěšné souhry mezi chromozomem a plazmidem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník příspěvků XXIII. konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-851-7

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

96-98

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

14. 6. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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