Plasmid-mediated colistin resistance in human and animal isolates of Escherichia coli: genomic surveillance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879731" target="_blank" >RIV/62157124:16270/21:43879731 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/21:43879731

Výsledek na webu

<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasmid-mediated colistin resistance in human and animal isolates of Escherichia coli: genomic surveillance

Popis výsledku v původním jazyce

Increasing occurrence of colistin-resistant bacteria represents a global concern since colistin is lastline antibiotic for the treatment of severe infections. Recent discovery of mobile colistin resistance mediated by mcr genes has raised extensive concerns worldwide. Within national surveillance of mcr genes in human clinical isolates in the Czech Republic a total of 1440 colistin-resistant isolates (MIC &gt;2 mg/l) were examined. At the same time more than 400 samples from food and food animals at slaughter were collected and selected for resistant isolates on agar media with colistin. The presence of mcr-1 to mcr-9 was tested by multiplex polymerase chain reaction (PCR). Isolates carrying mcr genes were subjected to antibiotic susceptibility testing and whole-genome sequencing to determined their resistance phenotype and genomic profiles. Conjugation experiments were used to test the transferability of mcr-carrying plasmids. The results showed a low prevalence of mcr genes in clinical samples (69/1440; 4,7 %) compared to isolates from veterinary sector (52/400; 13 %). Most isolates showed multi-drug resistance phenotype, belonged to various sequence types and carried mcr genes on conjugative plasmids.

Název v anglickém jazyce

Plasmid-mediated colistin resistance in human and animal isolates of Escherichia coli: genomic surveillance

Popis výsledku anglicky

Increasing occurrence of colistin-resistant bacteria represents a global concern since colistin is lastline antibiotic for the treatment of severe infections. Recent discovery of mobile colistin resistance mediated by mcr genes has raised extensive concerns worldwide. Within national surveillance of mcr genes in human clinical isolates in the Czech Republic a total of 1440 colistin-resistant isolates (MIC &gt;2 mg/l) were examined. At the same time more than 400 samples from food and food animals at slaughter were collected and selected for resistant isolates on agar media with colistin. The presence of mcr-1 to mcr-9 was tested by multiplex polymerase chain reaction (PCR). Isolates carrying mcr genes were subjected to antibiotic susceptibility testing and whole-genome sequencing to determined their resistance phenotype and genomic profiles. Conjugation experiments were used to test the transferability of mcr-carrying plasmids. The results showed a low prevalence of mcr genes in clinical samples (69/1440; 4,7 %) compared to isolates from veterinary sector (52/400; 13 %). Most isolates showed multi-drug resistance phenotype, belonged to various sequence types and carried mcr genes on conjugative plasmids.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV18-09-00605" target="_blank" >NV18-09-00605: Enterobacteriaceae rezistentní ke karbapenemům a kolistinu: koncept jednoho zdraví v hodnocení humánních a jiných zdrojů rizikových mechanizmů antimikrobiální rezistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník příspěvků XXIII. konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-851-7

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

114-116

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

14. 6. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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