Fitness effects of bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880035" target="_blank" >RIV/62157124:16270/22:43880035 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16810/22:43880035 RIV/00216208:11140/22:10445478 RIV/65269705:_____/22:00077567
Výsledek na webu
<a href="https://academic.oup.com/jac/article-abstract/77/11/2960/6650071?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/jac/article-abstract/77/11/2960/6650071?redirectedFrom=fulltext&login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dkac250" target="_blank" >10.1093/jac/dkac250</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Fitness effects of bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts
Popis výsledku v původním jazyce
Objectives To investigate the fitness effects of large bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts. Methods We selected five E. coli ST131 H30Rx isolates of diverse origin, each carrying an F2:A1:B- plasmid with the bla(CTX-M-15) gene. The plasmid was eliminated from each isolate by displacement using an incompatible curing plasmid, pMDP5_cureEC958. WGS was performed to obtain complete chromosome and plasmid sequences of original isolates and to detect chromosomal mutations in 'cured' clones. High-throughput competition assays were conducted to determine the relative fitness of cured clones compared with the corresponding original isolates. Results We were able to successfully eliminate the F2:A1:B- plasmids from all five original isolates using pMDP5_cureEC958. The F2:A1:B- plasmids produced non-significant fitness effects in three isolates and moderate reductions in relative fitness (3%-4%) in the two remaining isolates. Conclusions We conclude that F2:A1:B- plasmids pose low fitness costs in their E. coli ST131 H30Rx hosts. This plasmid-host fitness compatibility is likely to promote the maintenance of antibiotic resistance in this clinically important E. coli lineage.
Název v anglickém jazyce
Fitness effects of bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts
Popis výsledku anglicky
Objectives To investigate the fitness effects of large bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts. Methods We selected five E. coli ST131 H30Rx isolates of diverse origin, each carrying an F2:A1:B- plasmid with the bla(CTX-M-15) gene. The plasmid was eliminated from each isolate by displacement using an incompatible curing plasmid, pMDP5_cureEC958. WGS was performed to obtain complete chromosome and plasmid sequences of original isolates and to detect chromosomal mutations in 'cured' clones. High-throughput competition assays were conducted to determine the relative fitness of cured clones compared with the corresponding original isolates. Results We were able to successfully eliminate the F2:A1:B- plasmids from all five original isolates using pMDP5_cureEC958. The F2:A1:B- plasmids produced non-significant fitness effects in three isolates and moderate reductions in relative fitness (3%-4%) in the two remaining isolates. Conclusions We conclude that F2:A1:B- plasmids pose low fitness costs in their E. coli ST131 H30Rx hosts. This plasmid-host fitness compatibility is likely to promote the maintenance of antibiotic resistance in this clinically important E. coli lineage.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
1460-2091
Svazek periodika
77
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
4
Strana od-do
2960-2963
Kód UT WoS článku
000833570400001
EID výsledku v databázi Scopus
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