VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881579" target="_blank" >RIV/62157124:16270/24:43881579 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/24:10485279 RIV/62157124:16810/24:43881579 RIV/65269705:_____/24:00080420

Výsledek na webu

<a href="https://doi.org/10.1186/s12864-024-10780-7" target="_blank" >https://doi.org/10.1186/s12864-024-10780-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-024-10780-7" target="_blank" >10.1186/s12864-024-10780-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters

Popis výsledku v původním jazyce

Background Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment. Methods A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML. Results Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The blaVIM-1 gene was located within a class 1 integron that was carried by different F-type plasmids and one non-typeable plasmid. Furthermore, one of the isolates carried plasmid-borne colistin-resistance gene mcr-10, while in another isolate chromosomally located mcr-9 without colistin resistance phenotype was detected. In addition, the analysis of 685 publicly available E. asburiae genomes showed they frequently carry carbapenemase genes, highlighting the importance of this species in the emergence of resistance to last-line antibiotics. Conclusion Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.

Název v anglickém jazyce

VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters

Popis výsledku anglicky

Background Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment. Methods A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML. Results Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The blaVIM-1 gene was located within a class 1 integron that was carried by different F-type plasmids and one non-typeable plasmid. Furthermore, one of the isolates carried plasmid-borne colistin-resistance gene mcr-10, while in another isolate chromosomally located mcr-9 without colistin resistance phenotype was detected. In addition, the analysis of 685 publicly available E. asburiae genomes showed they frequently carry carbapenemase genes, highlighting the importance of this species in the emergence of resistance to last-line antibiotics. Conclusion Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NU20J-09-00040" target="_blank" >NU20J-09-00040: Celogenomové sekvenování a metagenomika jako nástroje k pochopení cest přenosu bakterií rezistentních k antibiotikům z nemocnic do prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC genomics

ISSN

1471-2164

e-ISSN

1471-2164

Svazek periodika

25

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

001317001200001

EID výsledku v databázi Scopus

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