Impact of haemogregarines on differential white blood cell count in tortoises of the genus Testudo

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Impact of haemogregarines on differential white blood cell count in tortoises of the genus Testudo

Popis výsledku v původním jazyce

The pathogenicity of blood parasites and their effect on the health status of their ectothermic host is a little explored. In our research, we focused on the impact of parasite Hemolivia mauritanica on the differential count of five types of leucocytes (lymphocytes, heterophils, azurophils, eosinophils and basophils). The blood samples originated from two species of tortoises, Testudo graeca and Testudo marginata. We have analyzed 206 blood smears overall, 25 samples originated from T. marginata and 181 originated from T. graeca. In these samples, we looked for positive individuals using light microscopy, calculated the differential leukocyte count and parasitemia. To determine whether infection itself has an influence on differential leucocyte count, we statistically evaluated leukocyte representation in infected and uninfected individuals. We also compared the impact of parasites on differential count of leucocytes with other categories, such as species of turtle, intensity of parasitemia, sex and age of the host, month and year of blood collection. Results of Wilcoxon ranksum tests showed us that in T. graeca, parasitemia and age had statistically significant influence on differential counts of leucocytes. In the turtle T. marginata, parasitemia had statistically significant influence on differential counts of basophils. After analyzing linear models to determine the effect of parasitemia on differential leukocyte count, we found statistically significant difference in counts of azurophils.

Název v anglickém jazyce

Impact of haemogregarines on differential white blood cell count in tortoises of the genus Testudo

Popis výsledku anglicky

The pathogenicity of blood parasites and their effect on the health status of their ectothermic host is a little explored. In our research, we focused on the impact of parasite Hemolivia mauritanica on the differential count of five types of leucocytes (lymphocytes, heterophils, azurophils, eosinophils and basophils). The blood samples originated from two species of tortoises, Testudo graeca and Testudo marginata. We have analyzed 206 blood smears overall, 25 samples originated from T. marginata and 181 originated from T. graeca. In these samples, we looked for positive individuals using light microscopy, calculated the differential leukocyte count and parasitemia. To determine whether infection itself has an influence on differential leucocyte count, we statistically evaluated leukocyte representation in infected and uninfected individuals. We also compared the impact of parasites on differential count of leucocytes with other categories, such as species of turtle, intensity of parasitemia, sex and age of the host, month and year of blood collection. Results of Wilcoxon ranksum tests showed us that in T. graeca, parasitemia and age had statistically significant influence on differential counts of leucocytes. In the turtle T. marginata, parasitemia had statistically significant influence on differential counts of basophils. After analyzing linear models to determine the effect of parasitemia on differential leukocyte count, we found statistically significant difference in counts of azurophils.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10613 - Zoology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXVI. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků

ISBN

978-80-7305-957-6

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

68-70

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

5. 6. 2024

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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