Purification of Leptin Receptor from Human Serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F04%3A00000307" target="_blank" >RIV/62157124:16370/04:00000307 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Purification of Leptin Receptor from Human Serum

Popis výsledku v původním jazyce

Leptin plays a significant role in the body weight regulation and it is frequently used as a precise indicator and modulator of adipose tissue volume. Based on the general pharmacology.... The soluble part of leptin receptor (OB-Re), which is anchored tothe membrane, is an antagonist of free, biologically active leptin. OB-Re influences significantly the leptin kinetic action and consequently its pharmacodynamic action.Material: the source of OB-Re was human serum; the mixture was prepared from 700 healthy volunteers of different age, sex and body weight.Methods: OB-Re was purified from human serum by immunoaffinity chromatography on the column with immobilized mouse monoclonal antibody. OB-Re was eluted from the column by using acid buffer (0.1M NaCl, 0.1M HCl, pH=2), the elution fraction was neutralized by PBS and The purity of the protein was confirmed in SDS PAGE and protein concentration measured by BCA method.We prepared total 5.5 ug of OB-Re, the protein concentration (BCA meth

Název v anglickém jazyce

Purification of Leptin Receptor from Human Serum

Popis výsledku anglicky

Leptin plays a significant role in the body weight regulation and it is frequently used as a precise indicator and modulator of adipose tissue volume. Based on the general pharmacology.... The soluble part of leptin receptor (OB-Re), which is anchored tothe membrane, is an antagonist of free, biologically active leptin. OB-Re influences significantly the leptin kinetic action and consequently its pharmacodynamic action.Material: the source of OB-Re was human serum; the mixture was prepared from 700 healthy volunteers of different age, sex and body weight.Methods: OB-Re was purified from human serum by immunoaffinity chromatography on the column with immobilized mouse monoclonal antibody. OB-Re was eluted from the column by using acid buffer (0.1M NaCl, 0.1M HCl, pH=2), the elution fraction was neutralized by PBS and The purity of the protein was confirmed in SDS PAGE and protein concentration measured by BCA method.We prepared total 5.5 ug of OB-Re, the protein concentration (BCA meth

Druh

D - Stať ve sborníku

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/NL7455" target="_blank" >NL7455: Sledování závislosti účinku na dávce a základních farmakokinetických parametrů u originálních látek s antioxidačním účinkem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Jahrestagung - Joint Meeting 2004

ISBN

3-00-014723-3

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

145

Název nakladatele

University of Regensburg

Místo vydání

Regensburg

Místo konání akce

Regensburg

Datum konání akce

6. 10. 2004

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

—