Separation of a mixture of luteolin-7-rutinoside and luteolin-7-neohesperidoside isolated from Ligustrum vulgare L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F09%3A00002093" target="_blank" >RIV/62157124:16370/09:00002093 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Separation of a mixture of luteolin-7-rutinoside and luteolin-7-neohesperidoside isolated from Ligustrum vulgare L.

Popis výsledku v původním jazyce

Simple and rapid TLC and column chromatographic methods have been established for separation of luteolin-7-rutinoside and luteolin-7-neohesperidoside on polyamide. A flavonoid mixture was isolated from the butanolic fraction of a methanolic extract of leaves of Ligustrum vulgare L. HPLC analysis of the isolated mixture showed the presence of two compounds in a ratio of approximately 1:1. TLC and column separation was achieved on polyamide with CHCl 3 -MeOH-2-butanone-acetylacetone 9:4:2:1 as mobile phase. After successful separation, the isolated compounds were identified by UV, MS, and NMR spectroscopy and by acid hydrolysis then comparison with authentic samples. Luteolin-7-neohesperidoside was isolated from Ligustrum vulgare L. for the first time.

Název v anglickém jazyce

Separation of a mixture of luteolin-7-rutinoside and luteolin-7-neohesperidoside isolated from Ligustrum vulgare L.

Popis výsledku anglicky

Simple and rapid TLC and column chromatographic methods have been established for separation of luteolin-7-rutinoside and luteolin-7-neohesperidoside on polyamide. A flavonoid mixture was isolated from the butanolic fraction of a methanolic extract of leaves of Ligustrum vulgare L. HPLC analysis of the isolated mixture showed the presence of two compounds in a ratio of approximately 1:1. TLC and column separation was achieved on polyamide with CHCl 3 -MeOH-2-butanone-acetylacetone 9:4:2:1 as mobile phase. After successful separation, the isolated compounds were identified by UV, MS, and NMR spectroscopy and by acid hydrolysis then comparison with authentic samples. Luteolin-7-neohesperidoside was isolated from Ligustrum vulgare L. for the first time.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Planar Chromatography, modern TLC

ISSN

0933-4173

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000269552900012

EID výsledku v databázi Scopus

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