Quercetin attenuates the ischemia reperfusion induced COX-2 and MPO expression in the small intestine mucosa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F17%3A43875997" target="_blank" >RIV/62157124:16370/17:43875997 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biopha.2017.08.038" target="_blank" >http://dx.doi.org/10.1016/j.biopha.2017.08.038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biopha.2017.08.038" target="_blank" >10.1016/j.biopha.2017.08.038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quercetin attenuates the ischemia reperfusion induced COX-2 and MPO expression in the small intestine mucosa

Popis výsledku v původním jazyce

Quercetin, the active substance of tea, fruits and vegetables, exerts a broad spectrum of pharmacological activities and is considered to have potential therapeutic application. The present study was designed to investigate the beneficial effect of quercetin against experimental ischemia-reperfusion (IR) injury of the small intestine in rats. Quercetin was administrated intraperitoneally 30 min before 1 h ischemia of superior mesenteric artery with following reperfusion periods lasting 1, 4 and 24 h. The male specific pathogen-free Charles River Wistar rats were used (n = 45). In acute phase, 4 h after start of reperfusion, the quercetin induced a significant decrease in mucosal injury index (p &lt; 0.05) accompanied by a significant decrease in cyclooxygenase-2 (COX-2) expression in the epithelial lining of the intestinal villi in comparison with the control group (p &lt; 0.01). In the epithelium of the intestinal glands, COX-2 expression resulting from IR injury significantly increased regardless quercetin application (in control group p &lt; 0.001; in quercetin group p &lt; 0.05), but in quercetin group, significant decrease in it during 24 h of reperfusion in a late phase of IR injury was detected (p &lt; 0.001). Based on morphology of COX-2 positive cells, the COX-2 positivity was found particularly in goblet cells of the intestinal villi epithelium and enteroendocrine cells respectively, in the glandular epithelium. We concluded that quercetin application attenuated mucosal damage from IR injury by inhibiting neutrophil infiltration which was demonstrated by a lower number of myeloperoxidase positive cells in the lamina propria during both phases of IR injury and the significant decrease in that in a late phase after 24 h of reperfusion (p &lt; 0.05).

Název v anglickém jazyce

Quercetin attenuates the ischemia reperfusion induced COX-2 and MPO expression in the small intestine mucosa

Popis výsledku anglicky

Quercetin, the active substance of tea, fruits and vegetables, exerts a broad spectrum of pharmacological activities and is considered to have potential therapeutic application. The present study was designed to investigate the beneficial effect of quercetin against experimental ischemia-reperfusion (IR) injury of the small intestine in rats. Quercetin was administrated intraperitoneally 30 min before 1 h ischemia of superior mesenteric artery with following reperfusion periods lasting 1, 4 and 24 h. The male specific pathogen-free Charles River Wistar rats were used (n = 45). In acute phase, 4 h after start of reperfusion, the quercetin induced a significant decrease in mucosal injury index (p &lt; 0.05) accompanied by a significant decrease in cyclooxygenase-2 (COX-2) expression in the epithelial lining of the intestinal villi in comparison with the control group (p &lt; 0.01). In the epithelium of the intestinal glands, COX-2 expression resulting from IR injury significantly increased regardless quercetin application (in control group p &lt; 0.001; in quercetin group p &lt; 0.05), but in quercetin group, significant decrease in it during 24 h of reperfusion in a late phase of IR injury was detected (p &lt; 0.001). Based on morphology of COX-2 positive cells, the COX-2 positivity was found particularly in goblet cells of the intestinal villi epithelium and enteroendocrine cells respectively, in the glandular epithelium. We concluded that quercetin application attenuated mucosal damage from IR injury by inhibiting neutrophil infiltration which was demonstrated by a lower number of myeloperoxidase positive cells in the lamina propria during both phases of IR injury and the significant decrease in that in a late phase after 24 h of reperfusion (p &lt; 0.05).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedicine &amp; pharmacotherapy

ISSN

0753-3322

e-ISSN

—

Svazek periodika

95

Číslo periodika v rámci svazku

NOV 2017

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

9

Strana od-do

346-354

Kód UT WoS článku

000417744400042

EID výsledku v databázi Scopus

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