Cognate PXR ligands tributyltin and triphenyltin isothiocyanates induce DNA crosslinks ? evoked apoptosis in human breast carcinoma MCF 7 and MDA-MB-231 cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F18%3A43877159" target="_blank" >RIV/62157124:16370/18:43877159 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cognate PXR ligands tributyltin and triphenyltin isothiocyanates induce DNA crosslinks ? evoked apoptosis in human breast carcinoma MCF 7 and MDA-MB-231 cell lines

Popis výsledku v původním jazyce

Two freshly synthesized triorganotin isothiocyanate compounds (commercially inavailable), cognate nuclear retinoid X receptor ligands, were tested in estrogen receptor positive MCF 7 and negative MDA-MB-231 human breast carcinoma cell lines. Cytotoxicity measured by MTT assay has shown tributyltin isothiocy to be more cytotoxic than triphenyltin isothiocyanate in MDA-MB-231 cell ine. Comet assay revealed the presence of crosslinks after the treatment wit derivatives. Differences in cytotoxicity of both compounds detected by FDA staining corresponded to the MTT data, indicating more pronounced effects in MCF 7 than in MDA-MB-231 cell line. Both derivatives caused apoptosis by intrinsic pathway, as shown by the mitochondrial membrane potential (MMP) depolarization an caspase-3/7 activation. The onset of caspase activation correlated with MMP dissipation and the tota cytotoxicity more than the amount of active caspases. In conclusion, our data suggest that the induced by tributyltin isothiocyanate and triphenyltin isothiocyanate treatment is the main cause of their cytotoxicity in cell lines studied. 13 1742/5000

Název v anglickém jazyce

Cognate PXR ligands tributyltin and triphenyltin isothiocyanates induce DNA crosslinks ? evoked apoptosis in human breast carcinoma MCF 7 and MDA-MB-231 cell lines

Popis výsledku anglicky

Two freshly synthesized triorganotin isothiocyanate compounds (commercially inavailable), cognate nuclear retinoid X receptor ligands, were tested in estrogen receptor positive MCF 7 and negative MDA-MB-231 human breast carcinoma cell lines. Cytotoxicity measured by MTT assay has shown tributyltin isothiocy to be more cytotoxic than triphenyltin isothiocyanate in MDA-MB-231 cell ine. Comet assay revealed the presence of crosslinks after the treatment wit derivatives. Differences in cytotoxicity of both compounds detected by FDA staining corresponded to the MTT data, indicating more pronounced effects in MCF 7 than in MDA-MB-231 cell line. Both derivatives caused apoptosis by intrinsic pathway, as shown by the mitochondrial membrane potential (MMP) depolarization an caspase-3/7 activation. The onset of caspase activation correlated with MMP dissipation and the tota cytotoxicity more than the amount of active caspases. In conclusion, our data suggest that the induced by tributyltin isothiocyanate and triphenyltin isothiocyanate treatment is the main cause of their cytotoxicity in cell lines studied. 13 1742/5000

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30107 - Medicinal chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů