Regulatory motifs in p53 biology - G-quadruplexes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F19%3A43878278" target="_blank" >RIV/62157124:16370/19:43878278 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Regulatory motifs in p53 biology - G-quadruplexes

Popis výsledku v původním jazyce

The focus of our work is the structure-specific binding of wild-type p53 and mutant p53 to DNA containing quadruplex-forming sequences in vitro and in cells and the effect on p53-driven transcription. CD analysis indicated that mutant p53 not only binds to G-quadruplex DNA, but also stabilizes G-quadruplex folds. Finally, we found that mutant p53 R273H preferentially binds to G/C-rich DNA around transcription start sites of many genes characterized by active chromatin marks and frequently associated with transcription-competent RNA polymerase II. We propose the role of G-quadruplexes as non-B DNA recognition motifs of wild-type and mutant p53 protein for transcriptional regulation of selected p53 target genes.

Název v anglickém jazyce

Regulatory motifs in p53 biology - G-quadruplexes

Popis výsledku anglicky

The focus of our work is the structure-specific binding of wild-type p53 and mutant p53 to DNA containing quadruplex-forming sequences in vitro and in cells and the effect on p53-driven transcription. CD analysis indicated that mutant p53 not only binds to G-quadruplex DNA, but also stabilizes G-quadruplex folds. Finally, we found that mutant p53 R273H preferentially binds to G/C-rich DNA around transcription start sites of many genes characterized by active chromatin marks and frequently associated with transcription-competent RNA polymerase II. We propose the role of G-quadruplexes as non-B DNA recognition motifs of wild-type and mutant p53 protein for transcriptional regulation of selected p53 target genes.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů