Escherichia coli isolates resistant to cephalosporins and quinolones from captive primates genetically resemble local human and food-source isolates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F18%3A43876811" target="_blank" >RIV/62157124:16810/18:43876811 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Escherichia coli isolates resistant to cephalosporins and quinolones from captive primates genetically resemble local human and food-source isolates

Popis výsledku v původním jazyce

The zoo environment, food sources and close proximity of the zookeepers may affect the prevalence of resistant bacteria in captive animals. The aim of the study was to evaluate possible transmission paths and dynamics of colonization of the primates with antibiotic-resistant bacteria in an artificial man-made ecosystem. During 2012, fecal samples of four common chimpanzees, their keepers and food samples (vegetables, granulated feed, and chickens) were tested in monthly periods in Ostrava zoo for presence of cefotaxime- and quinolone-resistant Escherichia coli. Five colonies obtained from selective cultivation were taken from each sample and tested for extended spectrum beta-lactamases (ESBLs), AmpC beta-lactamases and plasmid-mediated quinolone resistance (PMQR) genes by PCR. Pulsed-field gel electrophoresis was performed to assess the clonality and isolates from different samples showing indistinguishable profiles were subjected to whole-genome sequencing to analyze the genetic content and similarities within their genomes. Horizontal transfer of ESBL, AmpC and PMQR genes was tested using transformation and conjugation experiments, followed by further plasmid typing. A total of 121 isolates (58 cefotaxime-resistant/63 with reduced susceptibility to ciprofloxacin) were obtained from 37 samples. Only representatives of unique pulsotypes originating from the same sample were further analyzed. Of 25 cefotaxime-resistant isolates, 21 (84%) and three (12%) carried ESBL (blaCTX-M-1, blaCTX-M-15) and AmpC (blaCMY-2) genes, respectively. Two (9%) of 23 isolates with reduced susceptibility to ciprofloxacin harbored qnrS1 gene. Clinically relevant E. coli clones such as ST69 and ST117 have been identified. High genetic similarity of whole-genome content of 36 investigated isolates from all three-source groups, differing by low numbers of core-genome single nucleotide polymorphism, was detected. Moreover, long-term colonization of the primates by same isolates was demonstrated. The genes blaCTX-M and blaCMY-2 were carried by highly conjugative multiresistance IncI1 or IncK plasmids. IncI1/ST3 plasmids were the most prevalent and found in diverse E. coli STs and all source groups. Our study demonstrated extensive genetic commonality among isolates and resistance plasmids from chimpanzees, keepers, and food, suggesting possible zoonotic transmission of clinically important E. coli via the food chain, as well as an interspecies transmission between primates and humans.

Název v anglickém jazyce

Escherichia coli isolates resistant to cephalosporins and quinolones from captive primates genetically resemble local human and food-source isolates

Popis výsledku anglicky

The zoo environment, food sources and close proximity of the zookeepers may affect the prevalence of resistant bacteria in captive animals. The aim of the study was to evaluate possible transmission paths and dynamics of colonization of the primates with antibiotic-resistant bacteria in an artificial man-made ecosystem. During 2012, fecal samples of four common chimpanzees, their keepers and food samples (vegetables, granulated feed, and chickens) were tested in monthly periods in Ostrava zoo for presence of cefotaxime- and quinolone-resistant Escherichia coli. Five colonies obtained from selective cultivation were taken from each sample and tested for extended spectrum beta-lactamases (ESBLs), AmpC beta-lactamases and plasmid-mediated quinolone resistance (PMQR) genes by PCR. Pulsed-field gel electrophoresis was performed to assess the clonality and isolates from different samples showing indistinguishable profiles were subjected to whole-genome sequencing to analyze the genetic content and similarities within their genomes. Horizontal transfer of ESBL, AmpC and PMQR genes was tested using transformation and conjugation experiments, followed by further plasmid typing. A total of 121 isolates (58 cefotaxime-resistant/63 with reduced susceptibility to ciprofloxacin) were obtained from 37 samples. Only representatives of unique pulsotypes originating from the same sample were further analyzed. Of 25 cefotaxime-resistant isolates, 21 (84%) and three (12%) carried ESBL (blaCTX-M-1, blaCTX-M-15) and AmpC (blaCMY-2) genes, respectively. Two (9%) of 23 isolates with reduced susceptibility to ciprofloxacin harbored qnrS1 gene. Clinically relevant E. coli clones such as ST69 and ST117 have been identified. High genetic similarity of whole-genome content of 36 investigated isolates from all three-source groups, differing by low numbers of core-genome single nucleotide polymorphism, was detected. Moreover, long-term colonization of the primates by same isolates was demonstrated. The genes blaCTX-M and blaCMY-2 were carried by highly conjugative multiresistance IncI1 or IncK plasmids. IncI1/ST3 plasmids were the most prevalent and found in diverse E. coli STs and all source groups. Our study demonstrated extensive genetic commonality among isolates and resistance plasmids from chimpanzees, keepers, and food, suggesting possible zoonotic transmission of clinically important E. coli via the food chain, as well as an interspecies transmission between primates and humans.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů