Beta-lactamase producing Escherichia coli and antibiotic resistance genes in municipal and hospital wastewaters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F19%3A43878234" target="_blank" >RIV/62157124:16810/19:43878234 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Beta-lactamase producing Escherichia coli and antibiotic resistance genes in municipal and hospital wastewaters

Popis výsledku v původním jazyce

Wastewaters represent environment where sewage from different sources is interacting and are considered the hot spots of antimicrobial resistance (AMR). In this study, level of AMR in municipal (outflow/inflow to/from wastewater treatment plant (WWTP)), hospital (inflow/outflow to/from hospital WWTP and raw sewage from hospital) and river waters in the city of Brno (Czech Republic) was examined. Total vs cefotaxime (CTX)-resistant E. coli were enumerated in all samples. Based on phenotypic and genotypic results, representative CTX-resistant isolates and whole-community DNA were subjected to sequencing (Illumina) and bioinformatics analysis. In total 95.6% (n=158) of CTX-resistant E. coli were extended-spectrum beta-lactamase (ESBL) producers and were detected in all samples except the outflow from hospital WWTP. Most isolates were positive for gene blaCTX-M (98.7%; n=151) encoding ESBL. The metagenomic analysis showed high prevalence of pathogenic bacteria in hospital WWs and majority of non-pathogenic bacteria in municipal WWs, treated and river waters. Genes encoding resistance to aminoglycosides, beta-lactams and macrolides were frequently detected, however blaCTX-M was not found in this metagenomic dataset. The study confirmed municipal treated water as source of multi-resistant E. coli and AMR genes for the environment. Moreover, the combination of two different approaches brought complex view on AMR in water environment.

Název v anglickém jazyce

Beta-lactamase producing Escherichia coli and antibiotic resistance genes in municipal and hospital wastewaters

Popis výsledku anglicky

Wastewaters represent environment where sewage from different sources is interacting and are considered the hot spots of antimicrobial resistance (AMR). In this study, level of AMR in municipal (outflow/inflow to/from wastewater treatment plant (WWTP)), hospital (inflow/outflow to/from hospital WWTP and raw sewage from hospital) and river waters in the city of Brno (Czech Republic) was examined. Total vs cefotaxime (CTX)-resistant E. coli were enumerated in all samples. Based on phenotypic and genotypic results, representative CTX-resistant isolates and whole-community DNA were subjected to sequencing (Illumina) and bioinformatics analysis. In total 95.6% (n=158) of CTX-resistant E. coli were extended-spectrum beta-lactamase (ESBL) producers and were detected in all samples except the outflow from hospital WWTP. Most isolates were positive for gene blaCTX-M (98.7%; n=151) encoding ESBL. The metagenomic analysis showed high prevalence of pathogenic bacteria in hospital WWs and majority of non-pathogenic bacteria in municipal WWs, treated and river waters. Genes encoding resistance to aminoglycosides, beta-lactams and macrolides were frequently detected, however blaCTX-M was not found in this metagenomic dataset. The study confirmed municipal treated water as source of multi-resistant E. coli and AMR genes for the environment. Moreover, the combination of two different approaches brought complex view on AMR in water environment.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů