COMPARATIVE STUDY OF THE SEROLOGICAL TESTS USED FOR THE DIAGNOSIS OF Toxoplasma gondii INFECTION IN DOMESTIC PIGS

Kód výsledku v IS VaVaI

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RIV/62157124:16170/21:43879380

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

COMPARATIVE STUDY OF THE SEROLOGICAL TESTS USED FOR THE DIAGNOSIS OF Toxoplasma gondii INFECTION IN DOMESTIC PIGS

Popis výsledku v původním jazyce

Background. Toxoplasma gondii is the third most important foodborne parasite worldwide. It is estimated that one third of human global population is chronically infected and that the consumption of T. gondii cysts through raw or undercooked pork meat is one of the main infection sources for humans. Thus, surveillance is recommended to implement control measures and prevent the infection in domestic pigs. Nevertheless, the diagnostic performance of the techniques used is not always known, which can lead to misdiagnosis. Objectives. To accomplish a comparative study of the most widely used serological tests in pigs. Materials and Methods. Panels of sera from experimentally (n=202) and naturally (n=244) infected pigs were analysed by three commercial ELISAs (IDScreen?, PrioCHECKTM and Pigtype?). A tachyzoite based Western Blot was also employed with sera from experimentally infected pigs. The reference criterium used to classify sera as positive or negative was the result obtained by the majority of the tests and diagnostic performance was determined by TG-ROC analyses. Results. All ELISAs and the Western blot showed moderate to perfect agreement (k=0.632-0.903) with differences observed in Se and Sp values for the cut-offs suggested by the manufacturers (IDScreen?: AUC=1, Se=98 %, Sp=100 %; PrioCHECK TM: AUC=0.99, Se=100 %, Sp=75 %; Pigtype?: AUC=1, Se=99 %, Sp=99 %; WB: Se=92 %, Sp=100 %) with sera from experimental infections. However, the agreement notably decreased (k=0.363- 0.738) and the Se of IDScreen? was reduced (IDScreen?: AUC=0.96, Se=73 %, Sp=100 %; PrioCHECKTM: AUC=0.96, Se=100 %, Sp=72 %; Pigtype?: AUC=1, Se=98 %, Sp=97 %) with sera from naturally infected animals. Conclusion. The differences detected in the diagnostic performance among tests and regarding the panel of sera used evidence the need of harmonizing the serological tests routinely employed in pigs in order to obtain comparable and reliable results. Two additional in-house tests will be included in the comparative study (ELISA and IFI) and all tests will be readjusted if needed.

Název v anglickém jazyce

COMPARATIVE STUDY OF THE SEROLOGICAL TESTS USED FOR THE DIAGNOSIS OF Toxoplasma gondii INFECTION IN DOMESTIC PIGS

Popis výsledku anglicky

Background. Toxoplasma gondii is the third most important foodborne parasite worldwide. It is estimated that one third of human global population is chronically infected and that the consumption of T. gondii cysts through raw or undercooked pork meat is one of the main infection sources for humans. Thus, surveillance is recommended to implement control measures and prevent the infection in domestic pigs. Nevertheless, the diagnostic performance of the techniques used is not always known, which can lead to misdiagnosis. Objectives. To accomplish a comparative study of the most widely used serological tests in pigs. Materials and Methods. Panels of sera from experimentally (n=202) and naturally (n=244) infected pigs were analysed by three commercial ELISAs (IDScreen?, PrioCHECKTM and Pigtype?). A tachyzoite based Western Blot was also employed with sera from experimentally infected pigs. The reference criterium used to classify sera as positive or negative was the result obtained by the majority of the tests and diagnostic performance was determined by TG-ROC analyses. Results. All ELISAs and the Western blot showed moderate to perfect agreement (k=0.632-0.903) with differences observed in Se and Sp values for the cut-offs suggested by the manufacturers (IDScreen?: AUC=1, Se=98 %, Sp=100 %; PrioCHECK TM: AUC=0.99, Se=100 %, Sp=75 %; Pigtype?: AUC=1, Se=99 %, Sp=99 %; WB: Se=92 %, Sp=100 %) with sera from experimental infections. However, the agreement notably decreased (k=0.363- 0.738) and the Se of IDScreen? was reduced (IDScreen?: AUC=0.96, Se=73 %, Sp=100 %; PrioCHECKTM: AUC=0.96, Se=100 %, Sp=72 %; Pigtype?: AUC=1, Se=98 %, Sp=97 %) with sera from naturally infected animals. Conclusion. The differences detected in the diagnostic performance among tests and regarding the panel of sera used evidence the need of harmonizing the serological tests routinely employed in pigs in order to obtain comparable and reliable results. Two additional in-house tests will be included in the comparative study (ELISA and IFI) and all tests will be readjusted if needed.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů