Detection of clinically important beta-lactamases by using PCR
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F21%3A43879646" target="_blank" >RIV/62157124:16810/21:43879646 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16270/21:43879646
Výsledek na webu
<a href="https://academic.oup.com/femsle/article-abstract/368/11/fnab068/6294906?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/femsle/article-abstract/368/11/fnab068/6294906?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fnab068" target="_blank" >10.1093/femsle/fnab068</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Detection of clinically important beta-lactamases by using PCR
Popis výsledku v původním jazyce
Increasing antimicrobial resistance of nosocomial pathogens is becoming a serious threat to public health. To control the spread of this resistance, it is necessary to detect beta-lactamase-producing organisms in the clinical setting. The aims of the study were to design a PCR assay for rapid detection of clinically encountered beta-lactamase genes described in Enterobacteriaceae and Gram-negative non-fermenting bacteria. The functionality of proposed primers was verified using eight reference strains and 17 strains from our collection, which contained 29 different beta-lactamase genes. PCR products of the test strains were confirmed by Sanger sequencing. Sequence analysis was performed using bioinformatics software Geneious. Overall, 67 pairs of primers for detecting 12 members of the class C beta-lactamase family, 15 members of class A beta-lactamases, six gene families of subclass B1, one member each of subclasses B2, B3 and class D beta-lactamases were designed, of which 43 pairs were experimentally tested in vitro. All 29 beta-lactamase genes, including 10 oxacillinase subgroups, were correctly identified by PCR. The proposed set of primers should be able to specifically detect 99.7% of analyzed beta-lactamase subtypes and more than 79.8% of all described beta-lactamase genes.
Název v anglickém jazyce
Detection of clinically important beta-lactamases by using PCR
Popis výsledku anglicky
Increasing antimicrobial resistance of nosocomial pathogens is becoming a serious threat to public health. To control the spread of this resistance, it is necessary to detect beta-lactamase-producing organisms in the clinical setting. The aims of the study were to design a PCR assay for rapid detection of clinically encountered beta-lactamase genes described in Enterobacteriaceae and Gram-negative non-fermenting bacteria. The functionality of proposed primers was verified using eight reference strains and 17 strains from our collection, which contained 29 different beta-lactamase genes. PCR products of the test strains were confirmed by Sanger sequencing. Sequence analysis was performed using bioinformatics software Geneious. Overall, 67 pairs of primers for detecting 12 members of the class C beta-lactamase family, 15 members of class A beta-lactamases, six gene families of subclass B1, one member each of subclasses B2, B3 and class D beta-lactamases were designed, of which 43 pairs were experimentally tested in vitro. All 29 beta-lactamase genes, including 10 oxacillinase subgroups, were correctly identified by PCR. The proposed set of primers should be able to specifically detect 99.7% of analyzed beta-lactamase subtypes and more than 79.8% of all described beta-lactamase genes.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Fems microbiology letters
ISSN
0378-1097
e-ISSN
—
Svazek periodika
368
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
—
Kód UT WoS článku
000670887100004
EID výsledku v databázi Scopus
2-s2.0-85108386470