The natural cytotoxicity receptor genes in the family Felidae

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/62157124:16170/21:43879868

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The natural cytotoxicity receptor genes in the family Felidae

Popis výsledku v původním jazyce

Natural killer cells (NKC) play important roles in immune responses. Various NKC receptors (NKR) mediate multiple NKC activities. No single conservative model of NKR genes has been observed in mammals. As NKC in cats seem to be different from those of other mammals, analyses of NKR of the Felidae may bring new information on NKR evolution in mammals. Natural cytotoxicity receptors (NCR) represent a group of activating receptors encoded by genes NCR1, NCR2, and NCR3 expressed on immune cells. Their presence or functional characteristics may differ even in related species. The objective of this work was to characterize NCR genes in felids. Thirty-eight individuals of 15 felid species were analyzed. Based on bioinformatic analyses of the most recent genome assemblies and next-generation sequencing, potentially functional NCR1, NCR2 and NCR3 genes were found in all species analyzed. Similarities with the human NCR genes were 78%, 77% and 86%, respectively. Out of currently annotated mammalian NCR sequences, the most related were genes of families Hyaenidae and Herpestidae. Based on coding sequences (CDS), protein variants were inferred. Within the family, most interspecific differences in CDS were found in NCR1 (25, 1?4 per species), followed by NCR2 (23, 1?2 per species) and NCR3 (16, 1?2 per species). As for protein variants, 22 different NCR1 amino acid sequences (1?3 per species) were found, 21 (1?2 per species) for NCR2 and 12 (1?2 per species) for NCR3. Some protein variants were shared among species. For NCR1 and NCR2, their phylogenetic trees based on CDS reflected the current zoological taxonomy of felids. This finding was supported by selection analyses detecting neutral selection in both genes. Low variability of the NCR3 CDS did not allow a robust phylogeny reconstruction. Selection analysis detected purifying selection for NCR3. NCR1 showed most interspecific differences. A high level of within-species variation of NCR1 was also found in a group of 221 domestic cats. As NCR1 was associated with feline coronavirus shedding and considering differences in innate immunity observed among felid species, our data indicate a potential role of NCR1 in the interspecific variability.

Název v anglickém jazyce

The natural cytotoxicity receptor genes in the family Felidae

Popis výsledku anglicky

Natural killer cells (NKC) play important roles in immune responses. Various NKC receptors (NKR) mediate multiple NKC activities. No single conservative model of NKR genes has been observed in mammals. As NKC in cats seem to be different from those of other mammals, analyses of NKR of the Felidae may bring new information on NKR evolution in mammals. Natural cytotoxicity receptors (NCR) represent a group of activating receptors encoded by genes NCR1, NCR2, and NCR3 expressed on immune cells. Their presence or functional characteristics may differ even in related species. The objective of this work was to characterize NCR genes in felids. Thirty-eight individuals of 15 felid species were analyzed. Based on bioinformatic analyses of the most recent genome assemblies and next-generation sequencing, potentially functional NCR1, NCR2 and NCR3 genes were found in all species analyzed. Similarities with the human NCR genes were 78%, 77% and 86%, respectively. Out of currently annotated mammalian NCR sequences, the most related were genes of families Hyaenidae and Herpestidae. Based on coding sequences (CDS), protein variants were inferred. Within the family, most interspecific differences in CDS were found in NCR1 (25, 1?4 per species), followed by NCR2 (23, 1?2 per species) and NCR3 (16, 1?2 per species). As for protein variants, 22 different NCR1 amino acid sequences (1?3 per species) were found, 21 (1?2 per species) for NCR2 and 12 (1?2 per species) for NCR3. Some protein variants were shared among species. For NCR1 and NCR2, their phylogenetic trees based on CDS reflected the current zoological taxonomy of felids. This finding was supported by selection analyses detecting neutral selection in both genes. Low variability of the NCR3 CDS did not allow a robust phylogeny reconstruction. Selection analysis detected purifying selection for NCR3. NCR1 showed most interspecific differences. A high level of within-species variation of NCR1 was also found in a group of 221 domestic cats. As NCR1 was associated with feline coronavirus shedding and considering differences in innate immunity observed among felid species, our data indicate a potential role of NCR1 in the interspecific variability.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů