A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F13%3A50001708" target="_blank" >RIV/62690094:18470/13:50001708 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0278691513006480" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0278691513006480</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2013.09.010" target="_blank" >10.1016/j.fct.2013.09.010</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
Popis výsledku v původním jazyce
Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. W
Název v anglickém jazyce
A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
Popis výsledku anglicky
Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. W
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DN - Vliv životního prostředí na zdraví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Food and chemical toxicology
ISSN
0278-6915
e-ISSN
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Svazek periodika
62
Číslo periodika v rámci svazku
12.09.2013
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
5
Strana od-do
427-431
Kód UT WoS článku
000329960400056
EID výsledku v databázi Scopus
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