New Synthetic Pyrazine Carboxamide Derivatives as Potential Elicitors in Production of Secondary Metabolite in In vitro Cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F16%3A50004622" target="_blank" >RIV/62690094:18470/16:50004622 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/16:10327763

Výsledek na webu

<a href="http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=57;epage=62;aulast=T%F9mov%E1" target="_blank" >http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=57;epage=62;aulast=T%F9mov%E1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4103/0973-1296.176117" target="_blank" >10.4103/0973-1296.176117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New Synthetic Pyrazine Carboxamide Derivatives as Potential Elicitors in Production of Secondary Metabolite in In vitro Cultures

Popis výsledku v původním jazyce

Background: Silymarin, an active polyphenolic fraction of Silybum marianum, and high flavonoid content of Fagopyrum possess various interesting biological activities. The substituted pyrazine-2-carboxamides were previously used as effective elicitors of studied secondary metabolites. Objective: To study the effect of new synthetic pyrazine carboxamide derivatives, N-(4-chlorobenzyl)-5-tert-butylpyrazine-2-carboxamide (1) and 3-(3-((trifluoromethyl) benzyl) amino) pyrazine-2-carboxamide (2), on flavonolignan and flavonoid production in S. marianum and Fagopyrumes culentum in vitro cultures. Materials and Methods: Callus and suspension cultures were cultured on MS medium containing alpha-naphtaleneacetic acid or 2,4-D. Three elicitor concentrations for different exposure times were tested. Dried and powdered samples of callus and suspension cultures were extracted with methanol and analyzed by DAD-HPLC. Results: Compound 1 showed as a good elicitor of taxifolin production. The effect on silymarin complex was less visible with a maximum between 24 and 48 h after 3.292 x 10(-4) mol/L concentration. The detailed analysis showed that silychristin was the most abundant. Compound 2 was effective in rutin production only in callus culture with maximum 24 h and 168 h after application of 3.3756 x 10(-3) mol/L concentration and 48 and 72 h after 3.3756 x 10(-4) mol/L concentration. Conclusion: From the results of the performed experiments, it can be concluded that compound 1 shows to be suitable elicitor for enhanced production of taxifolin and silychristin in S. marianum, mainly when 3.292 x 10(-4) mol/L concentration was used, and compound 2 is suitable for increase rutin production in callus cultures and less appropriate for suspension cultures of F. esculentum.

Název v anglickém jazyce

New Synthetic Pyrazine Carboxamide Derivatives as Potential Elicitors in Production of Secondary Metabolite in In vitro Cultures

Popis výsledku anglicky

Background: Silymarin, an active polyphenolic fraction of Silybum marianum, and high flavonoid content of Fagopyrum possess various interesting biological activities. The substituted pyrazine-2-carboxamides were previously used as effective elicitors of studied secondary metabolites. Objective: To study the effect of new synthetic pyrazine carboxamide derivatives, N-(4-chlorobenzyl)-5-tert-butylpyrazine-2-carboxamide (1) and 3-(3-((trifluoromethyl) benzyl) amino) pyrazine-2-carboxamide (2), on flavonolignan and flavonoid production in S. marianum and Fagopyrumes culentum in vitro cultures. Materials and Methods: Callus and suspension cultures were cultured on MS medium containing alpha-naphtaleneacetic acid or 2,4-D. Three elicitor concentrations for different exposure times were tested. Dried and powdered samples of callus and suspension cultures were extracted with methanol and analyzed by DAD-HPLC. Results: Compound 1 showed as a good elicitor of taxifolin production. The effect on silymarin complex was less visible with a maximum between 24 and 48 h after 3.292 x 10(-4) mol/L concentration. The detailed analysis showed that silychristin was the most abundant. Compound 2 was effective in rutin production only in callus culture with maximum 24 h and 168 h after application of 3.3756 x 10(-3) mol/L concentration and 48 and 72 h after 3.3756 x 10(-4) mol/L concentration. Conclusion: From the results of the performed experiments, it can be concluded that compound 1 shows to be suitable elicitor for enhanced production of taxifolin and silychristin in S. marianum, mainly when 3.292 x 10(-4) mol/L concentration was used, and compound 2 is suitable for increase rutin production in callus cultures and less appropriate for suspension cultures of F. esculentum.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0022" target="_blank" >EE2.3.30.0022: Podpora vytváření, rozvoje a mobility kvalitních výzkumně-vývojových týmů na Univerzitě Karlově</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pharmacognosy magazine

ISSN

0973-1296

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

45

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

6

Strana od-do

57-62

Kód UT WoS článku

000370415100011

EID výsledku v databázi Scopus

—