Biological safety and tissue distribution of (16-mercaptohexadecyl) trimethylammonium bromide-modified cationic gold nanorods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F18%3A50014073" target="_blank" >RIV/62690094:18470/18:50014073 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/18:00494336 RIV/68378041:_____/18:00494336 RIV/68378050:_____/18:00494336 RIV/00179906:_____/18:10368634 RIV/68407700:21340/18:00318348

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0142961217307032?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0142961217307032?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biomaterials.2017.10.044" target="_blank" >10.1016/j.biomaterials.2017.10.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biological safety and tissue distribution of (16-mercaptohexadecyl) trimethylammonium bromide-modified cationic gold nanorods

Popis výsledku v původním jazyce

The exceptionally high cellular uptake of gold nanorods (GNRs) bearing cationic surfactants makes them a promising tool for biomedical applications. Given the known specific toxic and stress effects of some preparations of cationic nanoparticles, the purpose of this study was to evaluate, in an in vitro and in vivo in mouse, the potential harmful effects of GNRs coated with (16-mercaptohexadecyl)trimethylammo-nium bromide ((MTAB)GNRs). Interestingly, even after cellular accumulation of high amounts of (MTAB)GNRs sufficient for induction of photothermal effect, no genotoxicity (even after longer-term accumulation), induction of autophagy, destabilization of lysosomes (dominant organelles of their cellular destination), alterations of actin cytoskeleton, or in cell migration could be detected in vitro. In vivo, after intravenous administration, the majority of GNRs accumulated in mouse spleen followed by lungs and liver. Microscopic examination of the blood and spleen showed that GNRs interacted with white blood cells (mononuclear and polymorphonuclear leukocytes) and thrombocytes, arid were delivered to the spleen red pulp mainly as GNR-thrombocyte complexes. Importantly, no acute toxic effects of (MTAB)GNRs administered as 10 or 50 mu g of gold per mice, as well as no pathological changes after their high accumulation in the spleen were observed, indicating good tolerance of (MTAB)GNRs by living systems.

Název v anglickém jazyce

Biological safety and tissue distribution of (16-mercaptohexadecyl) trimethylammonium bromide-modified cationic gold nanorods

Popis výsledku anglicky

The exceptionally high cellular uptake of gold nanorods (GNRs) bearing cationic surfactants makes them a promising tool for biomedical applications. Given the known specific toxic and stress effects of some preparations of cationic nanoparticles, the purpose of this study was to evaluate, in an in vitro and in vivo in mouse, the potential harmful effects of GNRs coated with (16-mercaptohexadecyl)trimethylammo-nium bromide ((MTAB)GNRs). Interestingly, even after cellular accumulation of high amounts of (MTAB)GNRs sufficient for induction of photothermal effect, no genotoxicity (even after longer-term accumulation), induction of autophagy, destabilization of lysosomes (dominant organelles of their cellular destination), alterations of actin cytoskeleton, or in cell migration could be detected in vitro. In vivo, after intravenous administration, the majority of GNRs accumulated in mouse spleen followed by lungs and liver. Microscopic examination of the blood and spleen showed that GNRs interacted with white blood cells (mononuclear and polymorphonuclear leukocytes) and thrombocytes, arid were delivered to the spleen red pulp mainly as GNR-thrombocyte complexes. Importantly, no acute toxic effects of (MTAB)GNRs administered as 10 or 50 mu g of gold per mice, as well as no pathological changes after their high accumulation in the spleen were observed, indicating good tolerance of (MTAB)GNRs by living systems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomaterials

ISSN

0142-9612

e-ISSN

—

Svazek periodika

154

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

275-290

Kód UT WoS článku

000419538900022

EID výsledku v databázi Scopus

—