Non-targeted metabolomics reveals hormonal mechanisms regarding arbuscular mycorrhizal fungi- and Serendipita indica-mediated plant growth response in Camellia oleifera

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F24%3A50021642" target="_blank" >RIV/62690094:18470/24:50021642 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0304423824007003?dgcid=coauthor" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0304423824007003?dgcid=coauthor</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scienta.2024.113544" target="_blank" >10.1016/j.scienta.2024.113544</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Non-targeted metabolomics reveals hormonal mechanisms regarding arbuscular mycorrhizal fungi- and Serendipita indica-mediated plant growth response in Camellia oleifera

Popis výsledku v původním jazyce

Camellia oleifolia is an important oilseed woody plant that can be colonized by root-associated endophytic fungi, but the mechanism of how they promote growth improvement remains unclear. This study was to analyze the changes in biomass production, secondary metabolite profile, auxins, and cytokinins in C. oleifolia seedlings by four arbuscular mycorrhizal fungi (Diversispora versiformis, Rhizophagus intraradices, Funneliformis mosseae, and a mixture of the three above) and Serendipita indica. After 16 weeks of fungal inoculations, the root fungal colonization rate showed diverse changes, with S. indica (75 %) and R. intraradices (67 %) having relatively higher colonization rates. In addition, S. indica and R. intraradices significantly increased aboveground biomass, root biomass, and root surface area, whereas D. versiformis exhibited no significant effect. Non-targeted metabolomics of S. indica- and R. intraradices-inoculated roots detected a total of 802 metabolites, with 65 differential metabolites (33 up-regulated and 32 down-regulated) by S. indica and 163 differential metabolites (96 up-regulated and 67 down-regulated) by R. intraradices. The enriched metabolic pathways of these differential metabolites mainly included amino acid metabolism, secondary metabolites biosynthesis, and plant hormone biosynthesis in association with nine substances. The inoculation of endophytic fungi had varying effects on endogenous auxin and cytokinin levels, where leaf indole butyric acid and trans-zeatin levels were positively correlated with aboveground biomass, and root indole acetic acid, dihydrozeatin, and trans-zeatin levels were positively correlated with root biomass. It is concluded that root-associated endophytic fungi mediate the growth improvement by affecting auxins and cytokinins.

Název v anglickém jazyce

Non-targeted metabolomics reveals hormonal mechanisms regarding arbuscular mycorrhizal fungi- and Serendipita indica-mediated plant growth response in Camellia oleifera

Popis výsledku anglicky

Camellia oleifolia is an important oilseed woody plant that can be colonized by root-associated endophytic fungi, but the mechanism of how they promote growth improvement remains unclear. This study was to analyze the changes in biomass production, secondary metabolite profile, auxins, and cytokinins in C. oleifolia seedlings by four arbuscular mycorrhizal fungi (Diversispora versiformis, Rhizophagus intraradices, Funneliformis mosseae, and a mixture of the three above) and Serendipita indica. After 16 weeks of fungal inoculations, the root fungal colonization rate showed diverse changes, with S. indica (75 %) and R. intraradices (67 %) having relatively higher colonization rates. In addition, S. indica and R. intraradices significantly increased aboveground biomass, root biomass, and root surface area, whereas D. versiformis exhibited no significant effect. Non-targeted metabolomics of S. indica- and R. intraradices-inoculated roots detected a total of 802 metabolites, with 65 differential metabolites (33 up-regulated and 32 down-regulated) by S. indica and 163 differential metabolites (96 up-regulated and 67 down-regulated) by R. intraradices. The enriched metabolic pathways of these differential metabolites mainly included amino acid metabolism, secondary metabolites biosynthesis, and plant hormone biosynthesis in association with nine substances. The inoculation of endophytic fungi had varying effects on endogenous auxin and cytokinin levels, where leaf indole butyric acid and trans-zeatin levels were positively correlated with aboveground biomass, and root indole acetic acid, dihydrozeatin, and trans-zeatin levels were positively correlated with root biomass. It is concluded that root-associated endophytic fungi mediate the growth improvement by affecting auxins and cytokinins.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Horticulturae

ISSN

0304-4238

e-ISSN

1879-1018

Svazek periodika

337

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

"Article number: 113544"

Kód UT WoS článku

001298114600001

EID výsledku v databázi Scopus

2-s2.0-85201510552