1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F25%3A50021701" target="_blank" >RIV/62690094:18470/25:50021701 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00344-024-11481-x" target="_blank" >https://link.springer.com/article/10.1007/s00344-024-11481-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00344-024-11481-x" target="_blank" >10.1007/s00344-024-11481-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Popis výsledku v původním jazyce

Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction in silico between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (CaERS1-like, ETR2-like, ETR4-like and EIN4-like), increasing it in leaves (ETR1-like, ETR2-like, ETR4-like and CaCTR1-like); and increasing the expression of CaACO3-like (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.

Název v anglickém jazyce

1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Popis výsledku anglicky

Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction in silico between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (CaERS1-like, ETR2-like, ETR4-like and EIN4-like), increasing it in leaves (ETR1-like, ETR2-like, ETR4-like and CaCTR1-like); and increasing the expression of CaACO3-like (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of plant growth regulation

ISSN

0721-7595

e-ISSN

1435-8107

Svazek periodika

44

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

731-747

Kód UT WoS článku

001312262300003

EID výsledku v databázi Scopus

2-s2.0-85204132793