Phytochemicals Composition and Bioactivities of Fagopyrum esculentum Moench. Extract and Fractions With In Silico Insights

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F25%3A50023105" target="_blank" >RIV/62690094:18470/25:50023105 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202500241" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202500241</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cbdv.202500241" target="_blank" >10.1002/cbdv.202500241</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phytochemicals Composition and Bioactivities of Fagopyrum esculentum Moench. Extract and Fractions With In Silico Insights

Popis výsledku v původním jazyce

Leaves of Fagopyrum esculentum Moench were selected for their rich content of phytochemicals, offering potential biological activities. The aqueous residual fraction (AF) exhibited higher extraction yield (17.5%) compared with the other fractions. The highest content of phenols and flavonoids (84.27 +/- 2.7 mg/g GAE and 42.57 +/- 2.1 mg/g QUE, respectively) was found in AF among the different extracts. AF also showed the strong antioxidant properties (IC50 of DPPH: 61.43 +/- 0.58 mu g/mL; FRAP: 38.14 +/- 0.59 mu M Fe equivalents) and anti-inflammatory activity (IC50 = 69.87 +/- 1.26 mu g/mL). Furthermore, the alpha-amylase inhibition activity range varied from 19.39 +/- 2.11to 55.35 +/- 1.11% with an IC50 value of 40.59 +/- 0.03 mu g/mL, compared with standard metformin (IC50 = 19.88 +/- 91.51 mu g/mL). AF demonstrated significantly increased glucose uptake, range varied from 4.10 +/- 2.32% to 60.30 +/- 1.21%. AF are deemed non-toxic, as they preserve cell viability (HEK293) significantly above the IC50 threshold, with 75% of cells still viable at &lt;= 10 mu g/mL. LC-MS study of F. esculentum identified six major compounds (syringetin, pyrazinamide, glutaconic acid, 3-sulfolactic acid, gallic acid and kaempferol). Molecular docking study of kaempferol glucagon-like peptide-1 (GLP-1) showed good binding energies and interactions. Hence, F. esculentum possesses potential antidiabetic, antioxidant and cytotoxic activities.

Název v anglickém jazyce

Phytochemicals Composition and Bioactivities of Fagopyrum esculentum Moench. Extract and Fractions With In Silico Insights

Popis výsledku anglicky

Leaves of Fagopyrum esculentum Moench were selected for their rich content of phytochemicals, offering potential biological activities. The aqueous residual fraction (AF) exhibited higher extraction yield (17.5%) compared with the other fractions. The highest content of phenols and flavonoids (84.27 +/- 2.7 mg/g GAE and 42.57 +/- 2.1 mg/g QUE, respectively) was found in AF among the different extracts. AF also showed the strong antioxidant properties (IC50 of DPPH: 61.43 +/- 0.58 mu g/mL; FRAP: 38.14 +/- 0.59 mu M Fe equivalents) and anti-inflammatory activity (IC50 = 69.87 +/- 1.26 mu g/mL). Furthermore, the alpha-amylase inhibition activity range varied from 19.39 +/- 2.11to 55.35 +/- 1.11% with an IC50 value of 40.59 +/- 0.03 mu g/mL, compared with standard metformin (IC50 = 19.88 +/- 91.51 mu g/mL). AF demonstrated significantly increased glucose uptake, range varied from 4.10 +/- 2.32% to 60.30 +/- 1.21%. AF are deemed non-toxic, as they preserve cell viability (HEK293) significantly above the IC50 threshold, with 75% of cells still viable at &lt;= 10 mu g/mL. LC-MS study of F. esculentum identified six major compounds (syringetin, pyrazinamide, glutaconic acid, 3-sulfolactic acid, gallic acid and kaempferol). Molecular docking study of kaempferol glucagon-like peptide-1 (GLP-1) showed good binding energies and interactions. Hence, F. esculentum possesses potential antidiabetic, antioxidant and cytotoxic activities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CHEMISTRY &amp; BIODIVERSITY

ISSN

1612-1872

e-ISSN

1612-1880

Svazek periodika

22

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

22

Strana od-do

"Article Number: e00241"

Kód UT WoS článku

001555148300001

EID výsledku v databázi Scopus

2-s2.0-105013742414