Xylitol Production From Brewer's Spent Grain via Pichia fermentans Fermentation: Optimization, Scaling, and Isolation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F25%3A50023106" target="_blank" >RIV/62690094:18470/25:50023106 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27360/25:10258923

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1155/jfpp/9721088" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/jfpp/9721088</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/jfpp/9721088" target="_blank" >10.1155/jfpp/9721088</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Xylitol Production From Brewer's Spent Grain via Pichia fermentans Fermentation: Optimization, Scaling, and Isolation

Popis výsledku v původním jazyce

The primary aim of this study was to investigate the novel application of brewer&apos;s spent grain (BSG), a waste byproduct from the brewing industry, as sustainable and cost-effective feedstock for xylitol production using the yeast Pichia fermentans. The process encompassed fermentation optimization, scale-up, and then downstream processing to produce xylitol. Shake flask fermentation was employed to determine optimal conditions, evaluating key parameters including inoculum concentration (12.5%), feedstock (50%), pH (7.0), temperature (30 degrees C), incubation time (96 h), and agitation speed (150 RPM) with a maximum xylitol production of 32.74 g/L. The yield of xylitol increased to 34.57 g/L by scaling up in an 8-L bioreactor within an incubation time of 72 h. Downstream processing, including centrifugation, charcoal treatment, and ethanol purification was performed successfully, recovering xylitol crystals with a purity of 85.90%. Characterization using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) confirmed the purity and composition of crystals. This research highlights the economic and environmental advantages of utilizing BSG for xylitol production, offering a sustainable route over conventional substrates.

Název v anglickém jazyce

Xylitol Production From Brewer's Spent Grain via Pichia fermentans Fermentation: Optimization, Scaling, and Isolation

Popis výsledku anglicky

The primary aim of this study was to investigate the novel application of brewer&apos;s spent grain (BSG), a waste byproduct from the brewing industry, as sustainable and cost-effective feedstock for xylitol production using the yeast Pichia fermentans. The process encompassed fermentation optimization, scale-up, and then downstream processing to produce xylitol. Shake flask fermentation was employed to determine optimal conditions, evaluating key parameters including inoculum concentration (12.5%), feedstock (50%), pH (7.0), temperature (30 degrees C), incubation time (96 h), and agitation speed (150 RPM) with a maximum xylitol production of 32.74 g/L. The yield of xylitol increased to 34.57 g/L by scaling up in an 8-L bioreactor within an incubation time of 72 h. Downstream processing, including centrifugation, charcoal treatment, and ethanol purification was performed successfully, recovering xylitol crystals with a purity of 85.90%. Characterization using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) confirmed the purity and composition of crystals. This research highlights the economic and environmental advantages of utilizing BSG for xylitol production, offering a sustainable route over conventional substrates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF FOOD PROCESSING AND PRESERVATION

ISSN

0145-8892

e-ISSN

1745-4549

Svazek periodika

2025

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

"Article Number: 9721088"

Kód UT WoS článku

001438617900001

EID výsledku v databázi Scopus

2-s2.0-105000670675