Virucides in apiculture: persistence of surrogate enterovirus under simulated field conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62968335%3A_____%2F17%3AN0000007" target="_blank" >RIV/62968335:_____/17:N0000007 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.researchgate.net/publication/317850242_Virucides_in_apiculture_Persistence_of_surrogate_enterovirus_under_simulated_field_conditions" target="_blank" >https://www.researchgate.net/publication/317850242_Virucides_in_apiculture_Persistence_of_surrogate_enterovirus_under_simulated_field_conditions</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ps.4653" target="_blank" >10.1002/ps.4653</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Virucides in apiculture: persistence of surrogate enterovirus under simulated field conditions

Popis výsledku v původním jazyce

BACKGROUND: Honeybee viruses have been recognized as being among the most important factors leading to colony lossesworldwide. Colony food and faeces are regarded as possible sources of infectious viruses able to contaminate the environmentand equipment of apiaries. Thus, methods for elimination of viruses are required. No cell culture assay for testing the effect of disinfectants on honeybee viruses is yet available. Therefore, surrogate virus was employed for testing of the efficacy of iodophor- and peracetic acid-based disinfectants in combination with six organic contaminants at +6 ∘C and+22 ∘C. Moreover, we evaluated the persistence of the surrogate in honey at +6 ∘C, +22 ∘C, and +50 ∘C. RESULTS: Iodophor-based disinfectant showed a maximum reduction of virus titre of 3.4 log10. Peracetic acid reduced the titre (≥4 log10) only at 22∘C and without yeast extract/bovine serum albumin. After 25 days of incubation of the virus−honey mix, no decrease of virus titre was observed at +6 ∘C, whereas a significant reduction (3.5 log10) was found at +50 ∘C already after 1day. CONCLUSIONS: Both tested disinfectants can serve as appropriate virucides in apiaries. The effect of peracetic acid significantly depended on temperature and organic contaminants. The iodophor-based disinfectant showed a stable antiviral effect atdifferent temperatures and with different contaminants.

Název v anglickém jazyce

Virucides in apiculture: persistence of surrogate enterovirus under simulated field conditions

Popis výsledku anglicky

BACKGROUND: Honeybee viruses have been recognized as being among the most important factors leading to colony lossesworldwide. Colony food and faeces are regarded as possible sources of infectious viruses able to contaminate the environmentand equipment of apiaries. Thus, methods for elimination of viruses are required. No cell culture assay for testing the effect of disinfectants on honeybee viruses is yet available. Therefore, surrogate virus was employed for testing of the efficacy of iodophor- and peracetic acid-based disinfectants in combination with six organic contaminants at +6 ∘C and+22 ∘C. Moreover, we evaluated the persistence of the surrogate in honey at +6 ∘C, +22 ∘C, and +50 ∘C. RESULTS: Iodophor-based disinfectant showed a maximum reduction of virus titre of 3.4 log10. Peracetic acid reduced the titre (≥4 log10) only at 22∘C and without yeast extract/bovine serum albumin. After 25 days of incubation of the virus−honey mix, no decrease of virus titre was observed at +6 ∘C, whereas a significant reduction (3.5 log10) was found at +50 ∘C already after 1day. CONCLUSIONS: Both tested disinfectants can serve as appropriate virucides in apiaries. The effect of peracetic acid significantly depended on temperature and organic contaminants. The iodophor-based disinfectant showed a stable antiviral effect atdifferent temperatures and with different contaminants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1510113" target="_blank" >QJ1510113: Komplexní přístup k problematice virových chorob včel jako nástroj k podpoře a rozvoji včelařství v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the Society of Chemical Industry

ISSN

1934-9971

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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