Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F63839172%3A_____%2F25%3A10133804" target="_blank" >RIV/63839172:_____/25:10133804 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00140638

Výsledek na webu

<a href="https://doi.org/10.1016/j.pestbp.2025.106318" target="_blank" >https://doi.org/10.1016/j.pestbp.2025.106318</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pestbp.2025.106318" target="_blank" >10.1016/j.pestbp.2025.106318</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins

Popis výsledku v původním jazyce

Entomopathogenic nematodes (EPNs) are biological control agents that naturally kill insect pests, providing an eco-friendly alternative to chemical pesticides. Despite extensive research, the mechanisms behind the recovery process, where infective juveniles (IJs) transition to a parasitic state upon contact with the host, remain unclear. This study investigates the stimulatory effect of insect-derived materials on the recovery of Heterorhabditis bacteriophora IJs. Three materials from Galleria mellonella larvae-bioactive homogenates from live and frozen larvae, and heat-inactivated homogenate-were tested, along with non-host stimuli including filtered water and phosphate-buffered saline (PBS). While none of the materials induced complete recovery of IJs, all triggered the release of excreted/secreted products (ESPs), with consistent protein concentrations across treatments. However, mass spectrometry revealed significant differences in ESP protein composition. IJs exposed to PBS released the highest number of proteins, while bioactive homogenates induced the fewest. Proteins linked to host-parasite interactions, such as alpha-2-macroglobulins and trypsin inhibitor-like proteins, were more abundant in ESPs following exposure to insect-derived materials and PBS. Interestingly, nematodes exposed to water released a substantial number of proteins, comparable to stimulation by heat-inactivated homogenates, though their protein profiles were distinct, reflecting stress responses in the former and host-parasite interaction-related proteins in the latter. Our findings demonstrate that both host-derived and non-biological stimuli can trigger IJs recovery and ESPs release, underscoring the complexity of host-nematode interactions. These results provide novel insights into molecular mechanisms underlying H. bacteriophora parasitism and may contribute to optimizing biocontrol strategies through a better understanding of nematode activation and released ESPs.

Název v anglickém jazyce

Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins

Popis výsledku anglicky

Entomopathogenic nematodes (EPNs) are biological control agents that naturally kill insect pests, providing an eco-friendly alternative to chemical pesticides. Despite extensive research, the mechanisms behind the recovery process, where infective juveniles (IJs) transition to a parasitic state upon contact with the host, remain unclear. This study investigates the stimulatory effect of insect-derived materials on the recovery of Heterorhabditis bacteriophora IJs. Three materials from Galleria mellonella larvae-bioactive homogenates from live and frozen larvae, and heat-inactivated homogenate-were tested, along with non-host stimuli including filtered water and phosphate-buffered saline (PBS). While none of the materials induced complete recovery of IJs, all triggered the release of excreted/secreted products (ESPs), with consistent protein concentrations across treatments. However, mass spectrometry revealed significant differences in ESP protein composition. IJs exposed to PBS released the highest number of proteins, while bioactive homogenates induced the fewest. Proteins linked to host-parasite interactions, such as alpha-2-macroglobulins and trypsin inhibitor-like proteins, were more abundant in ESPs following exposure to insect-derived materials and PBS. Interestingly, nematodes exposed to water released a substantial number of proteins, comparable to stimulation by heat-inactivated homogenates, though their protein profiles were distinct, reflecting stress responses in the former and host-parasite interaction-related proteins in the latter. Our findings demonstrate that both host-derived and non-biological stimuli can trigger IJs recovery and ESPs release, underscoring the complexity of host-nematode interactions. These results provide novel insights into molecular mechanisms underlying H. bacteriophora parasitism and may contribute to optimizing biocontrol strategies through a better understanding of nematode activation and released ESPs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10616 - Entomology

Projekt

<a href="/cs/project/GA23-06457S" target="_blank" >GA23-06457S: Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi</a><br>

Návaznosti

—

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pesticide Biochemistry and Physiology

ISSN

0048-3575

e-ISSN

—

Svazek periodika

2025

Číslo periodika v rámci svazku

208

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1-14

Kód UT WoS článku

001425185300001

EID výsledku v databázi Scopus

2-s2.0-85217005822