Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F03%3A00007983" target="_blank" >RIV/65269705:_____/03:00007983 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2

Popis výsledku v původním jazyce

Both CD 8+ and CD 4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation snd proliferation of T cells require cellular interactions including adhesion,recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). Dcs werwobtained from peripheral blood mononuclear cells (PBMC) in the presence of IL ? 4 and GM-CSF. TNF ?á was added to induce maturation. A conjugate of myeloma idiotypic protein wih keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of IL-2 and IL-7. Autologous DCs were added to the lymphocyte on days 3,10, and17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in

Název v anglickém jazyce

Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2

Popis výsledku anglicky

Both CD 8+ and CD 4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation snd proliferation of T cells require cellular interactions including adhesion,recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). Dcs werwobtained from peripheral blood mononuclear cells (PBMC) in the presence of IL ? 4 and GM-CSF. TNF ?á was added to induce maturation. A conjugate of myeloma idiotypic protein wih keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of IL-2 and IL-7. Autologous DCs were added to the lymphocyte on days 3,10, and17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NC6763" target="_blank" >NC6763: Využití monoklonálního imunoglobulinu k přípravě protinádorové vakcíny u nemocných s mnohočetným myelomem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neoplasma

ISSN

ISSN 0028-268

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

120-123

Kód UT WoS článku

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EID výsledku v databázi Scopus

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