Co-Expression of Cancer Stem Cell Markers Corresponds to a Pro-Tumorigenic Expression Profile in Pancreatic Adenocarcinoma
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F16%3A00065518" target="_blank" >RIV/65269705:_____/16:00065518 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00159816:_____/16:00065518 RIV/00216224:14310/16:00095874 RIV/00209805:_____/16:00078579
Výsledek na webu
<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159255" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159255</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0159255" target="_blank" >10.1371/journal.pone.0159255</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Co-Expression of Cancer Stem Cell Markers Corresponds to a Pro-Tumorigenic Expression Profile in Pancreatic Adenocarcinoma
Popis výsledku v původním jazyce
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific protumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.
Název v anglickém jazyce
Co-Expression of Cancer Stem Cell Markers Corresponds to a Pro-Tumorigenic Expression Profile in Pancreatic Adenocarcinoma
Popis výsledku anglicky
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific protumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS ONE
ISSN
1932-6203
e-ISSN
—
Svazek periodika
11
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
18
Strana od-do
"e0159255"
Kód UT WoS článku
000379579500112
EID výsledku v databázi Scopus
2-s2.0-84978537584