Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00067300" target="_blank" >RIV/65269705:_____/17:00067300 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/17:00097722

Výsledek na webu

<a href="https://ac.els-cdn.com/S1566070216302594/1-s2.0-S1566070216302594-main.pdf?_tid=913473ce-c88c-11e7-ae6b-00000aacb35e&acdnat=1510589352_7a9bb3a5d784b8dcfd4cc6a4653402a7" target="_blank" >https://ac.els-cdn.com/S1566070216302594/1-s2.0-S1566070216302594-main.pdf?_tid=913473ce-c88c-11e7-ae6b-00000aacb35e&acdnat=1510589352_7a9bb3a5d784b8dcfd4cc6a4653402a7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.autneu.2017.06.003" target="_blank" >10.1016/j.autneu.2017.06.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Popis výsledku v původním jazyce

Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.

Název v anglickém jazyce

Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Popis výsledku anglicky

Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Autonomic Neuroscience-Basic &amp; Clinical

ISSN

1566-0702

e-ISSN

—

Svazek periodika

206

Číslo periodika v rámci svazku

SEP 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

19-27

Kód UT WoS článku

000408178900003

EID výsledku v databázi Scopus

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