Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00067342" target="_blank" >RIV/65269705:_____/17:00067342 - isvavai.cz</a>

Výsledek na webu

<a href="https://ac.els-cdn.com/S2352396417302621/1-s2.0-S2352396417302621-main.pdf?_tid=ccaaeae6-cf85-11e7-81dc-00000aacb35e&acdnat=1511356103_0d6795e012f542d5d95df5b91b692c6f" target="_blank" >https://ac.els-cdn.com/S2352396417302621/1-s2.0-S2352396417302621-main.pdf?_tid=ccaaeae6-cf85-11e7-81dc-00000aacb35e&acdnat=1511356103_0d6795e012f542d5d95df5b91b692c6f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ebiom.2017.06.027" target="_blank" >10.1016/j.ebiom.2017.06.027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Popis výsledku v původním jazyce

Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting N500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotypingwas performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., &gt;= 2 alleles for &gt;= 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p &lt; 0.001). The distribution of the alleles was significantly different (p &lt; 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p &lt; 0.001) and Gt16 with Belgium (5/5, p &lt; 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype.

Název v anglickém jazyce

Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Popis výsledku anglicky

Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting N500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotypingwas performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., &gt;= 2 alleles for &gt;= 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p &lt; 0.001). The distribution of the alleles was significantly different (p &lt; 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p &lt; 0.001) and Gt16 with Belgium (5/5, p &lt; 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30302 - Epidemiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EBioMedicine

ISSN

2352-3964

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

AUG 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

155-163

Kód UT WoS článku

000410738600020

EID výsledku v databázi Scopus

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