Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00075960" target="_blank" >RIV/65269705:_____/17:00075960 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/18/12/2685" target="_blank" >https://www.mdpi.com/1422-0067/18/12/2685</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms18122685" target="_blank" >10.3390/ijms18122685</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM

Popis výsledku v původním jazyce

Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes&apos; maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR (&quot;before IVM&quot; group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses (&quot;after IVM&quot; group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes&apos; successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte&apos;s achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

Název v anglickém jazyce

Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM

Popis výsledku anglicky

Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes&apos; maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR (&quot;before IVM&quot; group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses (&quot;after IVM&quot; group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes&apos; successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte&apos;s achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

2685

Kód UT WoS článku

000418896700188

EID výsledku v databázi Scopus

2-s2.0-85037864484