MicroRNA-210 expression during childbirth and postpartum as potential biomarker of acute fetal hypoxia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00068759" target="_blank" >RIV/65269705:_____/18:00068759 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/18:00101847

Výsledek na webu

<a href="http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/" target="_blank" >http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

MicroRNA-210 expression during childbirth and postpartum as potential biomarker of acute fetal hypoxia

Popis výsledku v původním jazyce

Objective: To explore whether miR-210 expression can be used as a diagnostic and prognostic marker in acute fetal hypoxia. Methods: Whole blood samples of 29 women and their fetuses without hypoxia and 24 women and their fetuses with hypoxia were analysed in this study. Reverse transcrip-tion and quantitative real-time PCR were used to measure the expression of miR-210. Expression level differences between the control and hypoxic group in labour time and postpartum change fold were analysed by standard statistical tests. Results: We confirmed that miR-210 is significantly more upregulated in fetal blood with acute hypoxia when compared to maternal blood (P&lt;0.001). Furthermore, there was significant up-regulation in miR-210 level in the hypoxic group when compared to the control non-hypoxic group (P&lt;0.05) in both maternal and fetal blood. Our results did not confirm a significant difference in postpartum miR-210 clearance level 2, 8, 24 or 48 hours after labour. Conclusions: Our study confirmed miR-210 upregulation in the blood of pregnant women with acute fetal hypoxia at the time of labour compared to pregnant women without acute fetal hypoxia. Additional investigation should be done to determine miR-210 clearance.

Název v anglickém jazyce

MicroRNA-210 expression during childbirth and postpartum as potential biomarker of acute fetal hypoxia

Popis výsledku anglicky

Objective: To explore whether miR-210 expression can be used as a diagnostic and prognostic marker in acute fetal hypoxia. Methods: Whole blood samples of 29 women and their fetuses without hypoxia and 24 women and their fetuses with hypoxia were analysed in this study. Reverse transcrip-tion and quantitative real-time PCR were used to measure the expression of miR-210. Expression level differences between the control and hypoxic group in labour time and postpartum change fold were analysed by standard statistical tests. Results: We confirmed that miR-210 is significantly more upregulated in fetal blood with acute hypoxia when compared to maternal blood (P&lt;0.001). Furthermore, there was significant up-regulation in miR-210 level in the hypoxic group when compared to the control non-hypoxic group (P&lt;0.05) in both maternal and fetal blood. Our results did not confirm a significant difference in postpartum miR-210 clearance level 2, 8, 24 or 48 hours after labour. Conclusions: Our study confirmed miR-210 upregulation in the blood of pregnant women with acute fetal hypoxia at the time of labour compared to pregnant women without acute fetal hypoxia. Additional investigation should be done to determine miR-210 clearance.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/TE02000058" target="_blank" >TE02000058: Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů