The expression of T-cell specific microRNA associates with the transformation of indolent follicular lymphoma to agressive lymphomas

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00068764" target="_blank" >RIV/65269705:_____/18:00068764 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/18:00103393

Výsledek na webu

<a href="https://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/" target="_blank" >https://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The expression of T-cell specific microRNA associates with the transformation of indolent follicular lymphoma to agressive lymphomas

Popis výsledku v původním jazyce

Follicular lymphoma (FL) is the most common indolent non-Hodgkin lymphoma. Deregulation of small non-coding RNAs called miRNAs has an important role in the molecular pathogenesis of B cell lymphomas. Herein we describe connection of miRNAs expression with FL prognosis and its transformation to aggressive diffuse large B-cell lymphoma. Screening of 377 miRNAs in 8 patients with FL transformation identified 5 miRNAs with significantly different expression between FL and transformed FL. We chose one miRNA (miR-X1) for further analysis based on the results in a validation cohort (38 paired FL/tFL samples). Low miR-X1 levels associated with high FLIPI (p&lt;0.01), high clinical stage (p&lt;0.01) and shorter survival of FL patients (n=83; 10-year survival: &gt;90% vs &lt;55%; p&lt;0.01). miR-X1 was found to be expressed mainly in T-cells present in the tumour. Based on the described role of miR-X1 in T-cell biology and a known prognostic value of specific T-cell subsets in FL, we hypothesised that miR-X1 levels reflect the potential of malignant B-cells to interact with CD4+ T-cells within the context of tumour microenvironment. Currently we are investigating whether the miR-X1 downregulation might be causative for FL transformation. The prognostic scores used in FL (such as FLIPI, M7FLIPI, POD24PI) are based upon combination of several factors, their assessment is expensive, and requires high quality of biological material. The analyses of miR-X1 levels enables stratification of patients to prognostic groups with an accuracy comparable with FLIPI using the paraffin-embedded tissue samples, with minimal cost and short tour-around time.

Název v anglickém jazyce

The expression of T-cell specific microRNA associates with the transformation of indolent follicular lymphoma to agressive lymphomas

Popis výsledku anglicky

Follicular lymphoma (FL) is the most common indolent non-Hodgkin lymphoma. Deregulation of small non-coding RNAs called miRNAs has an important role in the molecular pathogenesis of B cell lymphomas. Herein we describe connection of miRNAs expression with FL prognosis and its transformation to aggressive diffuse large B-cell lymphoma. Screening of 377 miRNAs in 8 patients with FL transformation identified 5 miRNAs with significantly different expression between FL and transformed FL. We chose one miRNA (miR-X1) for further analysis based on the results in a validation cohort (38 paired FL/tFL samples). Low miR-X1 levels associated with high FLIPI (p&lt;0.01), high clinical stage (p&lt;0.01) and shorter survival of FL patients (n=83; 10-year survival: &gt;90% vs &lt;55%; p&lt;0.01). miR-X1 was found to be expressed mainly in T-cells present in the tumour. Based on the described role of miR-X1 in T-cell biology and a known prognostic value of specific T-cell subsets in FL, we hypothesised that miR-X1 levels reflect the potential of malignant B-cells to interact with CD4+ T-cells within the context of tumour microenvironment. Currently we are investigating whether the miR-X1 downregulation might be causative for FL transformation. The prognostic scores used in FL (such as FLIPI, M7FLIPI, POD24PI) are based upon combination of several factors, their assessment is expensive, and requires high quality of biological material. The analyses of miR-X1 levels enables stratification of patients to prognostic groups with an accuracy comparable with FLIPI using the paraffin-embedded tissue samples, with minimal cost and short tour-around time.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů