Magnetic-Activated Cell Sorting in Combination with Swim-Up Efficiency Improve Effectivity of Spermatozoa Separation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00069402" target="_blank" >RIV/65269705:_____/18:00069402 - isvavai.cz</a>

Výsledek na webu

<a href="https://content.sciendo.com/view/journals/acb/6/2/article-p55.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/6/2/article-p55.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2018-0010" target="_blank" >10.2478/acb-2018-0010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic-Activated Cell Sorting in Combination with Swim-Up Efficiency Improve Effectivity of Spermatozoa Separation

Popis výsledku v původním jazyce

Correct selection of spermatozoa before their using for an assisted reproductive techniques is one of the crucial step in therapy of human infertility. It was previously reported that male factor plays a major role in infertility. Basic semen analyses and standard methods for sperm selection in many cases does not eliminate sufficiently proportion of spermatozoa with genetics defects. Magnetic Activated Cell Sorting (MACS) is a selection method which reduce apoptotic sperm and improve sperm and embryo quality. The aim of our study was the comparison of swim-up method and MACS and their combination. We tested swim-up and MACS alone and treatment of spermatozoa in combination when was is first swim-up and second MACS and vice versa. In this study we evaluated sperm concentration, motility and their DNA integrity before and after separtion methods. On the basis of our results we recommend to use swim-up before MACS method. This approach brings better results in the sperm selection - lower proportion of spermatozoa with fragmented DNA and also it brings better gain of total spermatozoa usable for next IVF or ICSI methods.

Název v anglickém jazyce

Magnetic-Activated Cell Sorting in Combination with Swim-Up Efficiency Improve Effectivity of Spermatozoa Separation

Popis výsledku anglicky

Correct selection of spermatozoa before their using for an assisted reproductive techniques is one of the crucial step in therapy of human infertility. It was previously reported that male factor plays a major role in infertility. Basic semen analyses and standard methods for sperm selection in many cases does not eliminate sufficiently proportion of spermatozoa with genetics defects. Magnetic Activated Cell Sorting (MACS) is a selection method which reduce apoptotic sperm and improve sperm and embryo quality. The aim of our study was the comparison of swim-up method and MACS and their combination. We tested swim-up and MACS alone and treatment of spermatozoa in combination when was is first swim-up and second MACS and vice versa. In this study we evaluated sperm concentration, motility and their DNA integrity before and after separtion methods. On the basis of our results we recommend to use swim-up before MACS method. This approach brings better results in the sperm selection - lower proportion of spermatozoa with fragmented DNA and also it brings better gain of total spermatozoa usable for next IVF or ICSI methods.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

<a href="/cs/project/LTC18059" target="_blank" >LTC18059: Kultivační systém vhodný pro dlouhodobou kultivaci lidských folikulárních buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

6

Strana od-do

55-60

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85059741998