Expression of genes involved in neurogenesis and neuronal precursor cell proliferation as the new markers of human ovarian granulosa cells´ in vitro differentiational capability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00069930" target="_blank" >RIV/65269705:_____/18:00069930 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ivfbrno.cz/28-sympozium-asistovane-reprodukce-a-17-cesko-slovenska-konference-reprodukcni-gynekologie/t1169" target="_blank" >http://www.ivfbrno.cz/28-sympozium-asistovane-reprodukce-a-17-cesko-slovenska-konference-reprodukcni-gynekologie/t1169</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of genes involved in neurogenesis and neuronal precursor cell proliferation as the new markers of human ovarian granulosa cells´ in vitro differentiational capability

Popis výsledku v původním jazyce

Introduction: Human ovarian granulosa cells (hGCs), under physiological conditions, are responsible for a number of processes related to normal folliculogenesis and oogenesis. In recent years, it has been discovered that hGCs, in the conditions of long-term in vitro culture, have the properties of stem cells, being able differentiate into other cell types e.g. osteoblasts, chondrocytes, muscle cells, under the influence of appropriate factors. The new properties of hGCs can therefore be used to obtain differentiated cells applied, for example, in procedures related to the reconstruction of tissues after injuries. Materials and methods: Human granulosa cells came from women referred to the procedure of in vitro fertilization. hGCs were grown in long-term in vitro culture for 30 days. The total RNA was isolated after 1,7,15,30 days of in vitro culture. Affymetrix(R) microarrays were used to perform a complete transcriptome analysis. Results: Microarrays allowed to identify groups of genes related to the development of the nervous system and neurogenesis. Three ontological group: &quot;neurogenesis&quot;, &quot;neuronal precursor cells proliferation&quot; and &quot;nervous system development&quot; were chosen. The microarray results showed 131 genes from these three heatmaps. The highest change in expression was demonstrated by NTN4, CRIM1, NANOS1, FRY, CD9, ITGA3, ATP8B1, DFNA5, OXTR, and CLDN1. Conclusion: Presented results show that GCs gain new properties in long-term in vitro culture. These results suggest that GCs could possibly differentiate towards neuroblasts. Genes of highest expression could be the new gene markers of GC differentiation toward neuroblast.

Název v anglickém jazyce

Expression of genes involved in neurogenesis and neuronal precursor cell proliferation as the new markers of human ovarian granulosa cells´ in vitro differentiational capability

Popis výsledku anglicky

Introduction: Human ovarian granulosa cells (hGCs), under physiological conditions, are responsible for a number of processes related to normal folliculogenesis and oogenesis. In recent years, it has been discovered that hGCs, in the conditions of long-term in vitro culture, have the properties of stem cells, being able differentiate into other cell types e.g. osteoblasts, chondrocytes, muscle cells, under the influence of appropriate factors. The new properties of hGCs can therefore be used to obtain differentiated cells applied, for example, in procedures related to the reconstruction of tissues after injuries. Materials and methods: Human granulosa cells came from women referred to the procedure of in vitro fertilization. hGCs were grown in long-term in vitro culture for 30 days. The total RNA was isolated after 1,7,15,30 days of in vitro culture. Affymetrix(R) microarrays were used to perform a complete transcriptome analysis. Results: Microarrays allowed to identify groups of genes related to the development of the nervous system and neurogenesis. Three ontological group: &quot;neurogenesis&quot;, &quot;neuronal precursor cells proliferation&quot; and &quot;nervous system development&quot; were chosen. The microarray results showed 131 genes from these three heatmaps. The highest change in expression was demonstrated by NTN4, CRIM1, NANOS1, FRY, CD9, ITGA3, ATP8B1, DFNA5, OXTR, and CLDN1. Conclusion: Presented results show that GCs gain new properties in long-term in vitro culture. These results suggest that GCs could possibly differentiate towards neuroblasts. Genes of highest expression could be the new gene markers of GC differentiation toward neuroblast.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/NV18-01-00544" target="_blank" >NV18-01-00544: Bisfenoly jako významné endokrinní disruptory současnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů