Bisphenol S content in human follicular fluid and its effect on IVF outcomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00071202" target="_blank" >RIV/65269705:_____/19:00071202 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/humrep/article/34/Supplement_1/i1/5528444" target="_blank" >https://academic.oup.com/humrep/article/34/Supplement_1/i1/5528444</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/humrep/34.Supplement_1.1" target="_blank" >10.1093/humrep/34.Supplement_1.1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bisphenol S content in human follicular fluid and its effect on IVF outcomes

Popis výsledku v původním jazyce

Study question: Does presence of Bisphenol S in follicular fluid negatively affect early embryonic development and implantation rate of human embryos? Summary answer:Our results document that high concentrations of Bisphenol S in follicular fluid are associated with higher rates of embryos that stopped their development. What is known already: Human fertility is influenced by a range of chemicals permanently present in our environment. One of these chemical is Bisphenol S (BPS), which is commonly used. BPS is an endocrine disruptor that can influence variety of physiological processes. It has been documented that BPS can disrupt spindle formation in oocytes and thus negatively influence IVF and embryonic development in porcine oocytes. BPS is currently commonly used in manufacture of plastics and it is often chosen to replace Bisphenol A, which has been prohibited in some countries. A direct effect of BPS on meiotic maturation of human oocytes is still unclear. Study design, size, duration: In this study we evaluated relation between concentration of BPS in follicular fluid, IVF outcome and early embryonic development in human. A total of 38 patients aged 26 to 42 years were included in this prospective study from spring to autumn 2018. Their follicular fluid was collected during transvaginal follicle puncture. BPS content was determined by the LC-MS method (liquid chromatography-mass spectrometry). Participants/materials, setting, methods: Based on detection of BPS in follicular fluid, the set of patients was divided into two groups - with high content of BPS (&gt;5ng/ml) and with low content of BPS (&lt;5ng/ml). Number of oocytes collected, efficiency of IVF, number of embryos suitable for transfer or cryoconservation, number of abnormal embryos, quality of embryonic development, biochemical and clinical gravidity were evaluated in each of the groups. Statistical evaluation was performed using one-way ANOVA and chisquare test. Main results and the role of chance: The examination detected presence of BPS in 35 samples of follicular fluid (92.1 % of all samples analysed). In 19 samples, the concentration was higher than 5 ng/ml (50.0 % of samples analysed). Subsequently, individual parameters of IVF were assessed. There were no significant differences found between the groups of patients with high and low concentration of BPS in number of oocytes collected (low BPS 8.3 vs. high BPS 7.4), efficiency of fertilization (low BPS 80 % vs. 72 % high BPS) and number of embryos usable after in vitro cultivation (low BPS 4.4 vs. 4.2 high BPS). In the patients with high concentration of BPS, a significantly higher share of abnormal embryos after in vitro cultivation was found (low BPS 1.3 vs. high BPS 2.3). Similarly in the in rates of biochemical (low BPS 36.8 % vs. high BPS 21 %) and clinical (low BPS 31.5 % vs. high BPS 15.8 %) gravidities were not statistically significant differences. Limitations, reasons for caution: A limitation is the number of samples included and analysed in this study which slightly reduced the power of statistical analysis. Wider implications of the findings:In this study, BPS was detected inmore than 90 % of analysed follicular fluid samples. Our results indicate a potential negative effect of BPS presence for development of human embryos.

Název v anglickém jazyce

Bisphenol S content in human follicular fluid and its effect on IVF outcomes

Popis výsledku anglicky

Study question: Does presence of Bisphenol S in follicular fluid negatively affect early embryonic development and implantation rate of human embryos? Summary answer:Our results document that high concentrations of Bisphenol S in follicular fluid are associated with higher rates of embryos that stopped their development. What is known already: Human fertility is influenced by a range of chemicals permanently present in our environment. One of these chemical is Bisphenol S (BPS), which is commonly used. BPS is an endocrine disruptor that can influence variety of physiological processes. It has been documented that BPS can disrupt spindle formation in oocytes and thus negatively influence IVF and embryonic development in porcine oocytes. BPS is currently commonly used in manufacture of plastics and it is often chosen to replace Bisphenol A, which has been prohibited in some countries. A direct effect of BPS on meiotic maturation of human oocytes is still unclear. Study design, size, duration: In this study we evaluated relation between concentration of BPS in follicular fluid, IVF outcome and early embryonic development in human. A total of 38 patients aged 26 to 42 years were included in this prospective study from spring to autumn 2018. Their follicular fluid was collected during transvaginal follicle puncture. BPS content was determined by the LC-MS method (liquid chromatography-mass spectrometry). Participants/materials, setting, methods: Based on detection of BPS in follicular fluid, the set of patients was divided into two groups - with high content of BPS (&gt;5ng/ml) and with low content of BPS (&lt;5ng/ml). Number of oocytes collected, efficiency of IVF, number of embryos suitable for transfer or cryoconservation, number of abnormal embryos, quality of embryonic development, biochemical and clinical gravidity were evaluated in each of the groups. Statistical evaluation was performed using one-way ANOVA and chisquare test. Main results and the role of chance: The examination detected presence of BPS in 35 samples of follicular fluid (92.1 % of all samples analysed). In 19 samples, the concentration was higher than 5 ng/ml (50.0 % of samples analysed). Subsequently, individual parameters of IVF were assessed. There were no significant differences found between the groups of patients with high and low concentration of BPS in number of oocytes collected (low BPS 8.3 vs. high BPS 7.4), efficiency of fertilization (low BPS 80 % vs. 72 % high BPS) and number of embryos usable after in vitro cultivation (low BPS 4.4 vs. 4.2 high BPS). In the patients with high concentration of BPS, a significantly higher share of abnormal embryos after in vitro cultivation was found (low BPS 1.3 vs. high BPS 2.3). Similarly in the in rates of biochemical (low BPS 36.8 % vs. high BPS 21 %) and clinical (low BPS 31.5 % vs. high BPS 15.8 %) gravidities were not statistically significant differences. Limitations, reasons for caution: A limitation is the number of samples included and analysed in this study which slightly reduced the power of statistical analysis. Wider implications of the findings:In this study, BPS was detected inmore than 90 % of analysed follicular fluid samples. Our results indicate a potential negative effect of BPS presence for development of human embryos.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

<a href="/cs/project/NV18-01-00544" target="_blank" >NV18-01-00544: Bisfenoly jako významné endokrinní disruptory současnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů