Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3D culture system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00071862" target="_blank" >RIV/65269705:_____/19:00071862 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00113277

Výsledek na webu

<a href="http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf" target="_blank" >http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3D culture system

Popis výsledku v původním jazyce

Granulosa cells have several functions during follicular development; for example, theynurse the oocyte, regulate steroid hormones production and activity, and prevent oocyte against apoptosis. For these abilities, granulosa cells are crucial for many reproductive functions and are suitable for in vitro culture and testing. However, when granulosa cells are cultured in vitro as monolayers, they gradually lose most of their important functions. The aim of our study was to test the three dimensional (3D) culture system for human granulosa cells. For this purpose nanofibrous cellulose hydrogel GrowDex was used. The expression of pro-and anti-apoptotic factors in human granulosa cells was investigated with the target to verify the usability of the 3D system with GrowDex. Human granulosa cells denuded from oocytes were cultured for 16 days in 4-well NUNC culture dishes with GrowDex. Conventional 2D (monolayer) culture system was used asa control. After prolong 16 days culture the expression of pro-and anti-apoptoticmarkers; Bax and BCl 2 respectively, was examined by Western blotting. The cells cultured in 3D culture system expressed anti-apoptotic factor BCl 2 in higher amounts than cells cultured in the conventional 2D culture system. Besides, the expression ofpro-apoptotic factor Bax was lower in the 3D system than the expression in the conventional culture system.

Název v anglickém jazyce

Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3D culture system

Popis výsledku anglicky

Granulosa cells have several functions during follicular development; for example, theynurse the oocyte, regulate steroid hormones production and activity, and prevent oocyte against apoptosis. For these abilities, granulosa cells are crucial for many reproductive functions and are suitable for in vitro culture and testing. However, when granulosa cells are cultured in vitro as monolayers, they gradually lose most of their important functions. The aim of our study was to test the three dimensional (3D) culture system for human granulosa cells. For this purpose nanofibrous cellulose hydrogel GrowDex was used. The expression of pro-and anti-apoptotic factors in human granulosa cells was investigated with the target to verify the usability of the 3D system with GrowDex. Human granulosa cells denuded from oocytes were cultured for 16 days in 4-well NUNC culture dishes with GrowDex. Conventional 2D (monolayer) culture system was used asa control. After prolong 16 days culture the expression of pro-and anti-apoptoticmarkers; Bax and BCl 2 respectively, was examined by Western blotting. The cells cultured in 3D culture system expressed anti-apoptotic factor BCl 2 in higher amounts than cells cultured in the conventional 2D culture system. Besides, the expression ofpro-apoptotic factor Bax was lower in the 3D system than the expression in the conventional culture system.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/LTC18059" target="_blank" >LTC18059: Kultivační systém vhodný pro dlouhodobou kultivaci lidských folikulárních buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů