Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00072384" target="_blank" >RIV/65269705:_____/19:00072384 - isvavai.cz</a>

Výsledek na webu

<a href="https://content.sciendo.com/view/journals/acb/7/2/article-p66.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/7/2/article-p66.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2019-0009" target="_blank" >10.2478/acb-2019-0009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

Popis výsledku v původním jazyce

Oxygen metabolism is crucial in establishing successful pregnancy, since excessive amount of reactive oxygen species (ROS) may exert deleterious effects on the developing embryo. There are several defense mechanisms against oxidative stress in the female reproductive tract, including production of antioxidant enzymes by oviductal epithelial cells (OECs). Undoubtedly, OECs play major part in female fertility and may also serve as an in vitro model of the oviduct. Therefore, the aim of this study was to investigate the expression of genes involved in oxygen metabolism. We have isolated OECs from oviducts of crossbred gilts (n=45) and maintained their in vitro culture for 30 days, collecting their RNA at days 1, 7, 15 and 30. The gene expression was determined with the use of Affymetrix(R) Porcine Gene 1.1 ST Array Strip. Our results revealed 166 differentially expressed genes belonging to four ontology groups: .,cellular response to oxidative stress&quot;, &quot;cellular response to oxygen-containing compound&quot;, &quot;cellular response to oxygen levels&quot; and &quot;cellular response to reactive oxygen species&quot;, most of which are also involved in other major processes in the organism. However, our findings provide a valuable insight into porcine reproductive biology and may be utilized in optimization of assisted reproduction techniques. Running title: Genes involved in oxygen metabolism in oviductal epithelial cells

Název v anglickém jazyce

Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

Popis výsledku anglicky

Oxygen metabolism is crucial in establishing successful pregnancy, since excessive amount of reactive oxygen species (ROS) may exert deleterious effects on the developing embryo. There are several defense mechanisms against oxidative stress in the female reproductive tract, including production of antioxidant enzymes by oviductal epithelial cells (OECs). Undoubtedly, OECs play major part in female fertility and may also serve as an in vitro model of the oviduct. Therefore, the aim of this study was to investigate the expression of genes involved in oxygen metabolism. We have isolated OECs from oviducts of crossbred gilts (n=45) and maintained their in vitro culture for 30 days, collecting their RNA at days 1, 7, 15 and 30. The gene expression was determined with the use of Affymetrix(R) Porcine Gene 1.1 ST Array Strip. Our results revealed 166 differentially expressed genes belonging to four ontology groups: .,cellular response to oxidative stress&quot;, &quot;cellular response to oxygen-containing compound&quot;, &quot;cellular response to oxygen levels&quot; and &quot;cellular response to reactive oxygen species&quot;, most of which are also involved in other major processes in the organism. However, our findings provide a valuable insight into porcine reproductive biology and may be utilized in optimization of assisted reproduction techniques. Running title: Genes involved in oxygen metabolism in oviductal epithelial cells

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

66-76

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85074585697