Elevated epidermal growth factor receptor levels in Barett´s esophagus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00074795" target="_blank" >RIV/65269705:_____/21:00074795 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/ueg2.12144" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/ueg2.12144</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ueg2.12144" target="_blank" >10.1002/ueg2.12144</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Elevated epidermal growth factor receptor levels in Barett´s esophagus

Popis výsledku v původním jazyce

Introduction Gastroesophageal reflux disease (GERD) is a chronic gastrointestinal illness characterized by the reflux of gastric contents causing symptoms and/or complications such as reflux esophagitis (RE) or Barrett&apos;s esophagus (BE), the precursor of esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-5 Aims &amp; Methods The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with/without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with complications of GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with/without pathological changes were detected in neither of the patient groups (p&gt;0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes (p=0.034). Conclusion Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.

Název v anglickém jazyce

Elevated epidermal growth factor receptor levels in Barett´s esophagus

Popis výsledku anglicky

Introduction Gastroesophageal reflux disease (GERD) is a chronic gastrointestinal illness characterized by the reflux of gastric contents causing symptoms and/or complications such as reflux esophagitis (RE) or Barrett&apos;s esophagus (BE), the precursor of esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-5 Aims &amp; Methods The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with/without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with complications of GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with/without pathological changes were detected in neither of the patient groups (p&gt;0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes (p=0.034). Conclusion Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30200 - Clinical medicine

Projekt

<a href="/cs/project/NU20-03-00126" target="_blank" >NU20-03-00126: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů