Transposable Elements Activity in Hematological Malignances

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00075088" target="_blank" >RIV/65269705:_____/21:00075088 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.ceitec.eu/ceitec-phd-conference-2021/a3988" target="_blank" >https://www.ceitec.eu/ceitec-phd-conference-2021/a3988</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transposable Elements Activity in Hematological Malignances

Popis výsledku v původním jazyce

Transposable elements, or transposons, are sequence units present in eukaryotic genomes in a broad diversity of their structures. They have played an important role in evolution of many genomes. In the human genome, the vast majority of transposons is represented by retroelements (REs) that can generate new copies and integrate them in genomic DNA through RNA-mediated mechanisms. The long interspersed nucleic elements (LINE-1 or L1) utilize a &quot;copy-and -paste&quot; mechanisms to retrotranspose copies into new genomic loci. Active L1 retrotransposons also drive retrotransposition of other mobile DNAs, such as short interspersed elements (SINEs), a group including Alu elements, and SVAtransposons. RE activity is mostly silenced by various control mechanisms, however, genomic instability, especially in cancer cells, can be associated with RE reactivation. To our best knowledge, no systematic analysis has been performed to date to study RE activity in hematological malignancies. We aim to explore RE activity in different types of hematological malignancies. To identify tumor-specific RE insertions, we adopted a protocol based on high-throughput sequencing of amplicons containing a part of RE from Alu-Ya5, Alu-Yb8 or L1-HS families (the most active in humans), and its adjacent genomic region. In total, 118 samples (73 tumor and 45 normal DNA) from 49 patients and 4 samples from 2 control cell lines were analyzed. We found 26 candidate insertions in 13 tumor samples. These insertions will be validated using PCR and Sanger sequencing.

Název v anglickém jazyce

Transposable Elements Activity in Hematological Malignances

Popis výsledku anglicky

Transposable elements, or transposons, are sequence units present in eukaryotic genomes in a broad diversity of their structures. They have played an important role in evolution of many genomes. In the human genome, the vast majority of transposons is represented by retroelements (REs) that can generate new copies and integrate them in genomic DNA through RNA-mediated mechanisms. The long interspersed nucleic elements (LINE-1 or L1) utilize a &quot;copy-and -paste&quot; mechanisms to retrotranspose copies into new genomic loci. Active L1 retrotransposons also drive retrotransposition of other mobile DNAs, such as short interspersed elements (SINEs), a group including Alu elements, and SVAtransposons. RE activity is mostly silenced by various control mechanisms, however, genomic instability, especially in cancer cells, can be associated with RE reactivation. To our best knowledge, no systematic analysis has been performed to date to study RE activity in hematological malignancies. We aim to explore RE activity in different types of hematological malignancies. To identify tumor-specific RE insertions, we adopted a protocol based on high-throughput sequencing of amplicons containing a part of RE from Alu-Ya5, Alu-Yb8 or L1-HS families (the most active in humans), and its adjacent genomic region. In total, 118 samples (73 tumor and 45 normal DNA) from 49 patients and 4 samples from 2 control cell lines were analyzed. We found 26 candidate insertions in 13 tumor samples. These insertions will be validated using PCR and Sanger sequencing.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/GA19-11299S" target="_blank" >GA19-11299S: Úloha transpozibilních elementů u hematologických malignit</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů