Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00077793" target="_blank" >RIV/65269705:_____/22:00077793 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/22:00128888
Výsledek na webu
<a href="https://journals.muni.cz/CPR/article/view/33535/28610" target="_blank" >https://journals.muni.cz/CPR/article/view/33535/28610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5817/CPR2022-2-20" target="_blank" >10.5817/CPR2022-2-20</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
Popis výsledku v původním jazyce
In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.
Název v anglickém jazyce
Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
Popis výsledku anglicky
In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
<a href="/cs/project/EF16_013%2F0001708" target="_blank" >EF16_013/0001708: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Polar Reports
ISSN
1805-0689
e-ISSN
1805-0697
Svazek periodika
12
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
11
Strana od-do
269-279
Kód UT WoS článku
000935360100010
EID výsledku v databázi Scopus
—