Endocrine disruption of adipose physiology: Screening in SGBS cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F24%3A00080288" target="_blank" >RIV/65269705:_____/24:00080288 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00137604

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.4679" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.4679</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jat.4679" target="_blank" >10.1002/jat.4679</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Endocrine disruption of adipose physiology: Screening in SGBS cells

Popis výsledku v původním jazyce

The increasing use of industrial chemicals has raised concerns regarding exposure to endocrine-disrupting chemicals (EDCs), which interfere with developmental, reproductive and metabolic processes. Of particular concern is their interaction with adipose tissue, a vital component of the endocrine system regulating metabolic and hormonal functions. The SGBS (Simpson Golabi Behmel Syndrome) cell line, a well-established human-relevant model for adipocyte research, closely mimics native adipocytes&apos; properties. It responds to hormonal stimuli, undergoes adipogenesis and has been successfully used to study the impact of EDCs on adipose biology. In this study, we screened human exposure-relevant doses of various EDCs on the SGBS cell line to investigate their effects on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated; however, at higher doses, EDCs compromised cell viability, with cadmium chloride (CdCl2) showing the most pronounced effects. Intracellular lipid levels remained unaffected by EDCs, except for tributyltin (TBT), used as a positive control, which induced a significant increase. Analysis of adipogenesis-related protein expression revealed several effects, including downregulation of fatty acid-binding protein 4 (FABP4) by dibutyl phthalate, upregulation by CdCl2 and downregulation of perilipin 1 and FABP4 by perfluorooctanoic acid. Additionally, TBT induced dose-dependent upregulation of C/EBP alpha, perilipin 1 and FABP4 protein expression. These findings underscore the importance of employing appropriate models to study EDC-adipocyte interactions. Conclusions from this research could guide strategies to reduce the negative impacts of EDC exposure on adipose tissue. The increasing use of chemicals raises concerns about exposure to endocrine-disrupting chemicals (EDCs). This study used the Simpson Golabi Behmel Syndrome cell line, a human-relevant adipocyte model, to screen the effect of various EDCs on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated, and EDCs did not affect lipid levels except for tributyltin, which increased them and also upregulated FABP4, perilipin 1 and C/EBP alpha.

Název v anglickém jazyce

Endocrine disruption of adipose physiology: Screening in SGBS cells

Popis výsledku anglicky

The increasing use of industrial chemicals has raised concerns regarding exposure to endocrine-disrupting chemicals (EDCs), which interfere with developmental, reproductive and metabolic processes. Of particular concern is their interaction with adipose tissue, a vital component of the endocrine system regulating metabolic and hormonal functions. The SGBS (Simpson Golabi Behmel Syndrome) cell line, a well-established human-relevant model for adipocyte research, closely mimics native adipocytes&apos; properties. It responds to hormonal stimuli, undergoes adipogenesis and has been successfully used to study the impact of EDCs on adipose biology. In this study, we screened human exposure-relevant doses of various EDCs on the SGBS cell line to investigate their effects on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated; however, at higher doses, EDCs compromised cell viability, with cadmium chloride (CdCl2) showing the most pronounced effects. Intracellular lipid levels remained unaffected by EDCs, except for tributyltin (TBT), used as a positive control, which induced a significant increase. Analysis of adipogenesis-related protein expression revealed several effects, including downregulation of fatty acid-binding protein 4 (FABP4) by dibutyl phthalate, upregulation by CdCl2 and downregulation of perilipin 1 and FABP4 by perfluorooctanoic acid. Additionally, TBT induced dose-dependent upregulation of C/EBP alpha, perilipin 1 and FABP4 protein expression. These findings underscore the importance of employing appropriate models to study EDC-adipocyte interactions. Conclusions from this research could guide strategies to reduce the negative impacts of EDC exposure on adipose tissue. The increasing use of chemicals raises concerns about exposure to endocrine-disrupting chemicals (EDCs). This study used the Simpson Golabi Behmel Syndrome cell line, a human-relevant adipocyte model, to screen the effect of various EDCs on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated, and EDCs did not affect lipid levels except for tributyltin, which increased them and also upregulated FABP4, perilipin 1 and C/EBP alpha.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

<a href="/cs/project/EF17_043%2F0009632" target="_blank" >EF17_043/0009632: CETOCOEN Excellence</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Toxicology

ISSN

0260-437X

e-ISSN

1099-1263

Svazek periodika

44

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1784-1792

Kód UT WoS článku

001274671400001

EID výsledku v databázi Scopus

2-s2.0-85199411517