Bacteriome of the Middle Ear in Children and Young Adults With Cholesteatoma and Retraction Pocket: A Pilot Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F25%3A00081603" target="_blank" >RIV/65269705:_____/25:00081603 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00140801

Výsledek na webu

<a href="https://aao-hnsfjournals.onlinelibrary.wiley.com/doi/full/10.1002/oto2.70051" target="_blank" >https://aao-hnsfjournals.onlinelibrary.wiley.com/doi/full/10.1002/oto2.70051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/oto2.70051" target="_blank" >10.1002/oto2.70051</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bacteriome of the Middle Ear in Children and Young Adults With Cholesteatoma and Retraction Pocket: A Pilot Study

Popis výsledku v původním jazyce

ObjectiveChronic otitis media (COM) is a common middle ear disease in children and young adults. Dysfunction of the Eustachian tube and bacterial infection are the main causes. This pilot study aimed to describe and compare bacteriomes of the middle ear in children and young adults with serious forms of COM, such as cholesteatoma and retraction pocket (RP) of the tympanic membrane, with bacteriomes in healthy middle ears.Study DesignObservational study.SettingClinical practice in a tertiary center. From January 1, 2021 to August 31, 2022. Patients aged 0 to 20 years.MethodsIn this case-control study, middle ears were swabbed during surgery on children with cholesteatoma (N = 23) or RP (N = 26) and on children indicated for cochlear implant (N = 15, controls). Genomic DNA extraction was followed by creation of a 16S ribosomal DNA gene library and sequencing on a MiSeq instrument. Samples with relative abundance of at least one bacterial genus &gt;20% were considered positive for the specific genus.ResultsBacterial diversity was generally low in the middle ear samples from patients with COM, with DNA content from 1 or 2 bacteria usually dominating in the sample. A significant difference in positivity for one or more bacterial genera was observed between patients with cholesteatoma or RP (38.8%) versus patients indicated for cochlear implants (6.7%).ConclusionWhile middle ear bacteriomes in cases of cholesteatoma and RP differed from those of controls, findings in the 2 pathological conditions were similar. These results support the statement that the RP could be a precholesteatoma stage.

Název v anglickém jazyce

Bacteriome of the Middle Ear in Children and Young Adults With Cholesteatoma and Retraction Pocket: A Pilot Study

Popis výsledku anglicky

ObjectiveChronic otitis media (COM) is a common middle ear disease in children and young adults. Dysfunction of the Eustachian tube and bacterial infection are the main causes. This pilot study aimed to describe and compare bacteriomes of the middle ear in children and young adults with serious forms of COM, such as cholesteatoma and retraction pocket (RP) of the tympanic membrane, with bacteriomes in healthy middle ears.Study DesignObservational study.SettingClinical practice in a tertiary center. From January 1, 2021 to August 31, 2022. Patients aged 0 to 20 years.MethodsIn this case-control study, middle ears were swabbed during surgery on children with cholesteatoma (N = 23) or RP (N = 26) and on children indicated for cochlear implant (N = 15, controls). Genomic DNA extraction was followed by creation of a 16S ribosomal DNA gene library and sequencing on a MiSeq instrument. Samples with relative abundance of at least one bacterial genus &gt;20% were considered positive for the specific genus.ResultsBacterial diversity was generally low in the middle ear samples from patients with COM, with DNA content from 1 or 2 bacteria usually dominating in the sample. A significant difference in positivity for one or more bacterial genera was observed between patients with cholesteatoma or RP (38.8%) versus patients indicated for cochlear implants (6.7%).ConclusionWhile middle ear bacteriomes in cases of cholesteatoma and RP differed from those of controls, findings in the 2 pathological conditions were similar. These results support the statement that the RP could be a precholesteatoma stage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30206 - Otorhinolaryngology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

OTO Open

ISSN

2473-974X

e-ISSN

2473-974X

Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

"e70051"

Kód UT WoS článku

001421283300001

EID výsledku v databázi Scopus

2-s2.0-85218929900