Fibroblast Activation Protein Expressing Mesenchymal Cells Promote Glioblastoma Angiogenesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985807%3A_____%2F21%3A00544017" target="_blank" >RIV/67985807:_____/21:00544017 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00023884:_____/21:00008952 RIV/61383082:_____/21:00001039 RIV/00216208:11110/21:10429205 RIV/00064165:_____/21:10429205
Výsledek na webu
<a href="http://dx.doi.org/10.3390/cancers13133304" target="_blank" >http://dx.doi.org/10.3390/cancers13133304</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cancers13133304" target="_blank" >10.3390/cancers13133304</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Fibroblast Activation Protein Expressing Mesenchymal Cells Promote Glioblastoma Angiogenesis
Popis výsledku v původním jazyce
Fibroblast activation protein (FAP) is a membrane-bound protease that is upregulated in a wide range of tumours and viewed as a marker of tumour-promoting stroma. Previously, we demonstrated increased FAP expression in glioblastomas and described its localisation in cancer and stromal cells. In this study, we show that FAP+ stromal cells are mostly localised in the vicinity of activated CD105+ endothelial cells and their quantity positively correlates with glioblastoma vascularisation. FAP+ mesenchymal cells derived from human glioblastomas are non-tumorigenic and mostly lack the cytogenetic aberrations characteristic of glioblastomas. Conditioned media from these cells induce angiogenic sprouting and chemotaxis of endothelial cells and promote migration and growth of glioma cells. In a chorioallantoic membrane assay, co-application of FAP+ mesenchymal cells with glioma cells was associated with enhanced abnormal angiogenesis, as evidenced by an increased number of erythrocytes in vessel-like structures and higher occurrence of haemorrhages. FAP+ mesenchymal cells express proangiogenic factors, but in comparison to normal pericytes exhibit decreased levels of antiangiogenic molecules and an increased Angiopoietin 2/1 ratio. Our results show that FAP+ mesenchymal cells promote angiogenesis and glioma cell migration and growth by paracrine communication and in this manner, they may thus contribute to glioblastoma progression.
Název v anglickém jazyce
Fibroblast Activation Protein Expressing Mesenchymal Cells Promote Glioblastoma Angiogenesis
Popis výsledku anglicky
Fibroblast activation protein (FAP) is a membrane-bound protease that is upregulated in a wide range of tumours and viewed as a marker of tumour-promoting stroma. Previously, we demonstrated increased FAP expression in glioblastomas and described its localisation in cancer and stromal cells. In this study, we show that FAP+ stromal cells are mostly localised in the vicinity of activated CD105+ endothelial cells and their quantity positively correlates with glioblastoma vascularisation. FAP+ mesenchymal cells derived from human glioblastomas are non-tumorigenic and mostly lack the cytogenetic aberrations characteristic of glioblastomas. Conditioned media from these cells induce angiogenic sprouting and chemotaxis of endothelial cells and promote migration and growth of glioma cells. In a chorioallantoic membrane assay, co-application of FAP+ mesenchymal cells with glioma cells was associated with enhanced abnormal angiogenesis, as evidenced by an increased number of erythrocytes in vessel-like structures and higher occurrence of haemorrhages. FAP+ mesenchymal cells express proangiogenic factors, but in comparison to normal pericytes exhibit decreased levels of antiangiogenic molecules and an increased Angiopoietin 2/1 ratio. Our results show that FAP+ mesenchymal cells promote angiogenesis and glioma cell migration and growth by paracrine communication and in this manner, they may thus contribute to glioblastoma progression.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10103 - Statistics and probability
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cancers (Basel)
ISSN
2072-6694
e-ISSN
2072-6694
Svazek periodika
13
Číslo periodika v rámci svazku
13
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
24
Strana od-do
3304
Kód UT WoS článku
000670920100001
EID výsledku v databázi Scopus
2-s2.0-85108891582