Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F01%3A20010190" target="_blank" >RIV/67985823:_____/01:20010190 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx.

Popis výsledku v původním jazyce

Differentiation of cholinergic cell line NG108-15 induced by a combination of dibutyryl cyclic AMP (dbcAMP) and dexamethasone enhances the cholinergic phenotype of the cells more than that induced by either agent alone. We investigated the effect of treatment with dbcAMP and dexamethasone on potassium depolarization-evoked influx of calcium and its regulation by the muscarinic agonist carbachol. Depolarization of control cells and of cells differentiated in the presence of dbcAMP or dexamethasone alone,or in the combined presence of dbcAMP and dexamethasone induced, respectively, 2.2, 4.3, 2.7 and 10.7 fold increases of the resting [Ca2+]i. Dexamethasone alone and the combination of dbcAMP and dexamethasone augmented the number of muscarinic receptorsby 25 and 40, respectively. Inhibitors of N ((-conotoxin GVIA) or P/Q ((-agatoxin TK) calcium channels had no effect on Ca2+ influx in control cells whereas in cells differentiated in the combined presence of dbcAMP and dexamethasone the

Název v anglickém jazyce

Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx.

Popis výsledku anglicky

Differentiation of cholinergic cell line NG108-15 induced by a combination of dibutyryl cyclic AMP (dbcAMP) and dexamethasone enhances the cholinergic phenotype of the cells more than that induced by either agent alone. We investigated the effect of treatment with dbcAMP and dexamethasone on potassium depolarization-evoked influx of calcium and its regulation by the muscarinic agonist carbachol. Depolarization of control cells and of cells differentiated in the presence of dbcAMP or dexamethasone alone,or in the combined presence of dbcAMP and dexamethasone induced, respectively, 2.2, 4.3, 2.7 and 10.7 fold increases of the resting [Ca2+]i. Dexamethasone alone and the combination of dbcAMP and dexamethasone augmented the number of muscarinic receptorsby 25 and 40, respectively. Inhibitors of N ((-conotoxin GVIA) or P/Q ((-agatoxin TK) calcium channels had no effect on Ca2+ influx in control cells whereas in cells differentiated in the combined presence of dbcAMP and dexamethasone the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Brain Research

ISSN

0006-8993

e-ISSN

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Svazek periodika

910

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

134-141

Kód UT WoS článku

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EID výsledku v databázi Scopus

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